ProtParam
User-provided sequence:
10 20 30 40 50 60
MKRGESQAPD AIFGQSRAFA LSDSSVNPDV IEYLKSVRQE ALRTNAISIK NHMNLQKRTR
70 80 90 100 110 120
HKSSMYDDED EGALKRHAIS PSLIRLQRNV EIWVRWFNSV KATVLTNAYE FTGYEDETLD
130 140 150 160 170 180
LLLLFLKNYL EDMPSKCTTV EKIISVLNQH SFPEKAEEKE ENLQIDEEWA KNILVRLEKT
190 200 210 220 230 240
KIDSVEDVKK VITEGDKHEL VGYNQWFQYL INNEPQHTTF HEKITSKQLW VLIKYMSNTW
250 260 270 280 290 300
IKEIHKKGRH YRRLQDWLFY ILVHTPERVT AEYTSILRDL GKKCLELIQK KPVEAHENKI
310 320 330 340
TLPKEMAELN VEIPAAVENM TITELTVSVI AVNYGQKDLI E
References and
documentation are available.
Number of amino acids: 341
Molecular weight: 39859.60
Theoretical pI: 6.17
Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 47
Atomic composition:
Carbon C 1786
Hydrogen H 2834
Nitrogen N 482
Oxygen O 532
Sulfur S 9
Formula: C
1786H
2834N
482O
532S
9
Total number of atoms: 5643
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 56505
Abs 0.1% (=1 g/l) 1.418, assuming all pairs of Cys residues form cystines
Ext. coefficient 56380
Abs 0.1% (=1 g/l) 1.414, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 52.71
This classifies the protein as unstable.
Aliphatic index: 93.46
Grand average of hydropathicity (GRAVY): -0.525