ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKRGKGEKKA TKSRDGSGQV VPLTEPVVTA TGMVGTRSWI GGLFTRSNRR QDKAVDYTLS 

        70         80         90        100        110        120 
PLQEERLQRL QDRMVVPFDE TRPDHQESLK ALWNVAFPNV HLTGLVTEQW KEMGWQGPNP 

       130        140        150        160        170        180 
STDFRGCGFI ALENLLFSAR TYPVCFRRLL LKQRGDRAKW EYPFAVAGIN ISFMLIQMLD 

       190        200        210        220        230        240 
LQNNPKPKCL PGMNFLKLLE EDERAFDVLY CIAFAMMDAQ WLAMHASYME FNEVLQATRN 

       250        260 
QLERELSLDD IHRIQDLPAY NLLFQ 

References and documentation are available.

Number of amino acids: 265

Molecular weight: 30547.18

Theoretical pI: 7.67

Amino acid composition: 
Ala (A)  18	  6.8%
Arg (R)  20	  7.5%
Asn (N)  12	  4.5%
Asp (D)  15	  5.7%
Cys (C)   4	  1.5%
Gln (Q)  16	  6.0%
Glu (E)  17	  6.4%
Gly (G)  16	  6.0%
His (H)   4	  1.5%
Ile (I)   8	  3.0%
Leu (L)  32	 12.1%
Lys (K)  13	  4.9%
Met (M)  11	  4.2%
Phe (F)  14	  5.3%
Pro (P)  14	  5.3%
Ser (S)  11	  4.2%
Thr (T)  13	  4.9%
Trp (W)   6	  2.3%
Tyr (Y)   6	  2.3%
Val (V)  15	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 33

Atomic composition:

Carbon      C	      1364
Hydrogen    H	      2136
Nitrogen    N	       380
Oxygen      O	       388
Sulfur      S	        15

Formula: C1364H2136N380O388S15
Total number of atoms: 4283

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42190
Abs 0.1% (=1 g/l)   1.381, assuming all pairs of Cys residues form cystines


Ext. coefficient    41940
Abs 0.1% (=1 g/l)   1.373, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.42
This classifies the protein as unstable.



Aliphatic index: 82.08

Grand average of hydropathicity (GRAVY): -0.378