ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKRKTAEVKG EKERNSKQIS LEEDKIKGMF NPKIWDKTFQ DGLKKEIEDS QPYNWGTIHE 

        70         80         90        100        110        120 
LVNDDLLRAV RKEIETEIHF TKKETDIYRV NQSGDLANLS GLDWDDLSRL PNLFKLRQIL 

       130        140        150        160        170        180 
YSKQYRDFFG YVTKAGKLSG SKTDMSINTY TKGCHLLTHD DVIGSRRISF ILYLPDPDRK 

       190        200        210        220        230        240 
WKSHYGGGLR LFPSILPNVP HSDPSAKLVP QFNQIAFFKV LPGFSFHDVE EVKVDKHRLS 

       250        260        270        280        290        300 
IQGWYHIPQV GEEGYIPGEE EAWVRNNTST LAQIESNVLE DFEFPKDERN ILSFHEVKHF 

       310        320        330        340        350        360 
EKMLKGDAGA KTDNTPKESM TSVISDSVKL SEAEFTYLSQ YISPEHLSSK GIEKLQKQFV 

       370        380        390        400        410        420 
ENSSLQIESF LNDDKSELLK KVIKQKELEQ ECPYHSKDVK APWKTAIPPH KARYLYIDGK 

       430        440        450        460        470        480 
EYRNFQTEAD ILEALNNNDL PNFQFTKDAI KIISDASGNS RENNFDAELA LIDLAVFHKS 

       490        500        510        520        530        540 
TIFKKYLALL TSLCPVSEQI LIRRFRPGMD FTLATKCRFN ELLKSNPDII DAVLEGTLCL 

       550        560        570        580        590        600 
TPSAGWESGE LGGYELYMMD DDEDNKQYLK EDVEDASVYR ADDSGDSVLI NDPPAWNTFN 

       610        620        630        640 
LVLRDESVLE FVKYVSWSAK SSRWDVKMKW DVKSCDEDGQ EDEA 

References and documentation are available.

Number of amino acids: 644

Molecular weight: 74041.44

Theoretical pI: 5.23

Amino acid composition: 
Ala (A)  30	  4.7%
Arg (R)  25	  3.9%
Asn (N)  31	  4.8%
Asp (D)  54	  8.4%
Cys (C)   6	  0.9%
Gln (Q)  22	  3.4%
Glu (E)  55	  8.5%
Gly (G)  32	  5.0%
His (H)  15	  2.3%
Ile (I)  38	  5.9%
Leu (L)  62	  9.6%
Lys (K)  60	  9.3%
Met (M)   9	  1.4%
Phe (F)  31	  4.8%
Pro (P)  27	  4.2%
Ser (S)  53	  8.2%
Thr (T)  27	  4.2%
Trp (W)  12	  1.9%
Tyr (Y)  22	  3.4%
Val (V)  33	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 109
Total number of positively charged residues (Arg + Lys): 85

Atomic composition:

Carbon      C	      3318
Hydrogen    H	      5138
Nitrogen    N	       874
Oxygen      O	      1018
Sulfur      S	        15

Formula: C3318H5138N874O1018S15
Total number of atoms: 10363

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    99155
Abs 0.1% (=1 g/l)   1.339, assuming all pairs of Cys residues form cystines


Ext. coefficient    98780
Abs 0.1% (=1 g/l)   1.334, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.18
This classifies the protein as unstable.



Aliphatic index: 80.08

Grand average of hydropathicity (GRAVY): -0.621