ProtParam
User-provided sequence:
10 20 30 40 50 60
MKRLTERVHH EEQESEEVVW VKRDDARSAG KLPIVFSRNY AVRFGGLERL HPFDAAKGKH
70 80 90 100 110 120
IHKLLCAQLQ LDDGSFYEPT ELTKDQLRRI HTREYLKSLR WSMNVACIAE VPLMAFVPNR
130 140 150 160 170 180
YIQRSYLRPM RFQAAGSILA GKLALDYGWA INLGGGFHHC CSYRGGGFCP YADISLLIVR
190 200 210 220 230 240
LFEQEPFRVR RIMIVDLDAH QGNGHERDFN NVAAVYIFDM YNAFVYPRDH VAKESIRCAV
250 260 270 280 290 300
ELRNYTEDGF YLRQLKRCLM QSLAEFRPDM VVYNAGTDVL EGDPLGNLAI SAEGVIERDR
310 320 330 340
LVFSTFRALG IPVVMLLSGG YLKASAGVIT DSIVNLRLQG LLN
References and
documentation are available.
Number of amino acids: 343
Molecular weight: 39064.01
Theoretical pI: 8.40
Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 43
Atomic composition:
Carbon C 1750
Hydrogen H 2741
Nitrogen N 497
Oxygen O 488
Sulfur S 16
Formula: C
1750H
2741N
497O
488S
16
Total number of atoms: 5492
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 39225
Abs 0.1% (=1 g/l) 1.004, assuming all pairs of Cys residues form cystines
Ext. coefficient 38850
Abs 0.1% (=1 g/l) 0.995, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.16
This classifies the protein as unstable.
Aliphatic index: 94.96
Grand average of hydropathicity (GRAVY): -0.133