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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKRLTERVHH EEQESEEVVW VKRDDARSAG KLPIVFSRNY AVRFGGLERL HPFDAAKGKH

70 80 90 100 110 120
IHKLLCAQLQ LDDGSFYEPT ELTKDQLRRI HTREYLKSLR WSMNVACIAE VPLMAFVPNR

130 140 150 160 170 180
YIQRSYLRPM RFQAAGSILA GKLALDYGWA INLGGGFHHC CSYRGGGFCP YADISLLIVR

190 200 210 220 230 240
LFEQEPFRVR RIMIVDLDAH QGNGHERDFN NVAAVYIFDM YNAFVYPRDH VAKESIRCAV

250 260 270 280 290 300
ELRNYTEDGF YLRQLKRCLM QSLAEFRPDM VVYNAGTDVL EGDPLGNLAI SAEGVIERDR

310 320 330 340
LVFSTFRALG IPVVMLLSGG YLKASAGVIT DSIVNLRLQG LLN


References and documentation are available.

Number of amino acids: 343 Molecular weight: 39064.01 Theoretical pI: 8.40
Amino acid composition: 
Ala (A) 28 8.2% Arg (R) 31 9.0% Asn (N) 13 3.8% Asp (D) 19 5.5% Cys (C) 7 2.0% Gln (Q) 11 3.2% Glu (E) 21 6.1% Gly (G) 26 7.6% His (H) 11 3.2% Ile (I) 18 5.2% Leu (L) 39 11.4% Lys (K) 12 3.5% Met (M) 9 2.6% Phe (F) 17 5.0% Pro (P) 12 3.5% Ser (S) 17 5.0% Thr (T) 8 2.3% Trp (W) 3 0.9% Tyr (Y) 15 4.4% Val (V) 26 7.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 40 Total number of positively charged residues (Arg + Lys): 43 Atomic composition: Carbon C 1750 Hydrogen H 2741 Nitrogen N 497 Oxygen O 488 Sulfur S 16 Formula: C1750H2741N497O488S16 Total number of atoms: 5492 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 39225 Abs 0.1% (=1 g/l) 1.004, assuming all pairs of Cys residues form cystines Ext. coefficient 38850 Abs 0.1% (=1 g/l) 0.995, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 47.16 This classifies the protein as unstable. Aliphatic index: 94.96 Grand average of hydropathicity (GRAVY): -0.133