ProtParam

User-provided sequence:

        10         20         30         40         50 
MKRQKRDKLG RAHSNGYQAG LGGKTKEQCP YQNIDAKSQW LGGWRDAISD RNLGLFK 

References and documentation are available.

Number of amino acids: 57

Molecular weight: 6478.34

Theoretical pI: 10.10

Amino acid composition: 
Ala (A)   4	  7.0%
Arg (R)   5	  8.8%
Asn (N)   3	  5.3%
Asp (D)   4	  7.0%
Cys (C)   1	  1.8%
Gln (Q)   5	  8.8%
Glu (E)   1	  1.8%
Gly (G)   8	 14.0%
His (H)   1	  1.8%
Ile (I)   2	  3.5%
Leu (L)   5	  8.8%
Lys (K)   7	 12.3%
Met (M)   1	  1.8%
Phe (F)   1	  1.8%
Pro (P)   1	  1.8%
Ser (S)   3	  5.3%
Thr (T)   1	  1.8%
Trp (W)   2	  3.5%
Tyr (Y)   2	  3.5%
Val (V)   0	  0.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 5
Total number of positively charged residues (Arg + Lys): 12

Atomic composition:

Carbon      C	       281
Hydrogen    H	       449
Nitrogen    N	        91
Oxygen      O	        82
Sulfur      S	         2

Formula: C281H449N91O82S2
Total number of atoms: 905

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13980
Abs 0.1% (=1 g/l)   2.158, assuming all pairs of Cys residues form cystines


Ext. coefficient    13980
Abs 0.1% (=1 g/l)   2.158, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.95
This classifies the protein as stable.



Aliphatic index: 54.91

Grand average of hydropathicity (GRAVY): -1.200