ProtParam

User-provided sequence:

        10         20         30         40         50 
MKRQKRDRLD RAFSKGFQAG IGGRSKELCP YANLDSRSQW LGGWREGVDG RVNGLFNK 

References and documentation are available.

Number of amino acids: 58

Molecular weight: 6614.49

Theoretical pI: 10.57

Amino acid composition: 
Ala (A)   3	  5.2%
Arg (R)   8	 13.8%
Asn (N)   3	  5.2%
Asp (D)   4	  6.9%
Cys (C)   1	  1.7%
Gln (Q)   3	  5.2%
Glu (E)   2	  3.4%
Gly (G)   9	 15.5%
His (H)   0	  0.0%
Ile (I)   1	  1.7%
Leu (L)   5	  8.6%
Lys (K)   5	  8.6%
Met (M)   1	  1.7%
Phe (F)   3	  5.2%
Pro (P)   1	  1.7%
Ser (S)   4	  6.9%
Thr (T)   0	  0.0%
Trp (W)   2	  3.4%
Tyr (Y)   1	  1.7%
Val (V)   2	  3.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 6
Total number of positively charged residues (Arg + Lys): 13

Atomic composition:

Carbon      C	       287
Hydrogen    H	       457
Nitrogen    N	        95
Oxygen      O	        82
Sulfur      S	         2

Formula: C287H457N95O82S2
Total number of atoms: 923

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12490
Abs 0.1% (=1 g/l)   1.888, assuming all pairs of Cys residues form cystines


Ext. coefficient    12490
Abs 0.1% (=1 g/l)   1.888, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 58.79
This classifies the protein as unstable.



Aliphatic index: 55.52

Grand average of hydropathicity (GRAVY): -1.016