ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKRSRGSSDS LSGFLPIRHS TTDKQISPRP TTTGFLYSGA GDYSQMFDAL EDDGSLEDLG 

        70         80         90        100        110        120 
GVGHASSTAA EKKRRLGVEQ VKALEKNFEI DNKLEPERKV KLAQELGLQP RQVAIWFQNR 

       130        140        150        160        170        180 
RARWKTKQLE RDYGVLKSNF DALKRNRDSL QRDNDSLLGQ IKELKAKLNV EGVKGIEENG 

       190        200        210        220        230        240 
ALKAVEANQS VMANNEVLEL SHRSPSPPPH IPTDAPTSEL AFEMFSIFPR TENFRDDPAD 

       250        260        270        280        290        300 
SSDSSAVLNE EYSPNTVEAA GAVAATTVEM STMGCFSQFV KMEEHEDLFS GEEACKLFAD 

       310 
NEQWYCSDQW NS 

References and documentation are available.

Number of amino acids: 312

Molecular weight: 34790.63

Theoretical pI: 5.02

Amino acid composition: 
Ala (A)  26	  8.3%
Arg (R)  18	  5.8%
Asn (N)  16	  5.1%
Asp (D)  21	  6.7%
Cys (C)   3	  1.0%
Gln (Q)  14	  4.5%
Glu (E)  30	  9.6%
Gly (G)  19	  6.1%
His (H)   5	  1.6%
Ile (I)   8	  2.6%
Leu (L)  27	  8.7%
Lys (K)  20	  6.4%
Met (M)   7	  2.2%
Phe (F)  14	  4.5%
Pro (P)  14	  4.5%
Ser (S)  31	  9.9%
Thr (T)  14	  4.5%
Trp (W)   4	  1.3%
Tyr (Y)   5	  1.6%
Val (V)  16	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1510
Hydrogen    H	      2372
Nitrogen    N	       430
Oxygen      O	       495
Sulfur      S	        10

Formula: C1510H2372N430O495S10
Total number of atoms: 4817

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    29575
Abs 0.1% (=1 g/l)   0.850, assuming all pairs of Cys residues form cystines


Ext. coefficient    29450
Abs 0.1% (=1 g/l)   0.846, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 56.17
This classifies the protein as unstable.



Aliphatic index: 66.96

Grand average of hydropathicity (GRAVY): -0.707