ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKRSTLLSLD AFAKTEEDVR VRTRAGGLIT LSCILTTLFL LVNEWGQFNS VVTRPQLVVD 

        70         80         90        100        110        120 
RDRHAKLELN MDVTFPSMPC DLVNLDIMDD SGEMQLDILD AGFTMSRLNS EGRPVGDATE 

       130        140        150        160        170        180 
LHVGGNGDGT APVNNDPNYC GPCYGAKDQS QNENLAQEEK VCCQDCDAVR SAYLEAGWAF 

       190        200        210        220        230        240 
FDGKNIEQCE REGYVSKINE HLNEGCRIKG SAQINRIQGN LHFAPGKPYQ NAYGHFHDTS 

       250        260        270        280        290        300 
LYDKTSNLNF NHIINHLSFG KPIQSHSKLL GNDKRHGGAV VATSPLDGRQ VFPDRNTHFH 

       310        320        330        340        350        360 
QFSYFAKIVP TRYEYLDNVV IETAQFSATF HSRPLAGGRD KDHPNTLHVR GGIPGMFVFF 

       370        380        390        400        410 
EMSPLKVINK EQHGQTWSGF ILNCITSIGG VLAVGTVMDK LFYKAQRSIW GKKSQ 

References and documentation are available.

Number of amino acids: 415

Molecular weight: 46235.14

Theoretical pI: 6.35

Amino acid composition: 
Ala (A)  24	  5.8%
Arg (R)  21	  5.1%
Asn (N)  27	  6.5%
Asp (D)  27	  6.5%
Cys (C)  10	  2.4%
Gln (Q)  19	  4.6%
Glu (E)  20	  4.8%
Gly (G)  37	  8.9%
His (H)  16	  3.9%
Ile (I)  20	  4.8%
Leu (L)  35	  8.4%
Lys (K)  21	  5.1%
Met (M)   9	  2.2%
Phe (F)  22	  5.3%
Pro (P)  17	  4.1%
Ser (S)  26	  6.3%
Thr (T)  22	  5.3%
Trp (W)   4	  1.0%
Tyr (Y)  11	  2.7%
Val (V)  27	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 42

Atomic composition:

Carbon      C	      2037
Hydrogen    H	      3157
Nitrogen    N	       581
Oxygen      O	       615
Sulfur      S	        19

Formula: C2037H3157N581O615S19
Total number of atoms: 6409

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    39015
Abs 0.1% (=1 g/l)   0.844, assuming all pairs of Cys residues form cystines


Ext. coefficient    38390
Abs 0.1% (=1 g/l)   0.830, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.52
This classifies the protein as stable.



Aliphatic index: 76.34

Grand average of hydropathicity (GRAVY): -0.400