ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKRVALLAVG SFNPPTIAHL RMLEVARSHL ETINTQVVEG IMSPVADSYN NKPTLIKSNF 

        70         80         90        100        110        120 
RIQMVRAATK SSDWIRADDW ECTRTTWTRT IDVLRHHREL VQEKFGSDVG MMLVVGGDVV 

       130        140        150        160        170        180 
DSFTRILPDG SNLWNSSDIR TIITEFGLIV LSREGSNPLN TIQSMPAISE FCDRIIQVKD 

       190        200        210        220 
EVCPSGVSST RLRAAIMNKK SIKYSTPDEV INFIRENNLY QKI 

References and documentation are available.

Number of amino acids: 223

Molecular weight: 25167.93

Theoretical pI: 8.46

Amino acid composition: 
Ala (A)  11	  4.9%
Arg (R)  17	  7.6%
Asn (N)  13	  5.8%
Asp (D)  13	  5.8%
Cys (C)   3	  1.3%
Gln (Q)   6	  2.7%
Glu (E)  12	  5.4%
Gly (G)  10	  4.5%
His (H)   4	  1.8%
Ile (I)  21	  9.4%
Leu (L)  16	  7.2%
Lys (K)  10	  4.5%
Met (M)   8	  3.6%
Phe (F)   7	  3.1%
Pro (P)   9	  4.0%
Ser (S)  21	  9.4%
Thr (T)  16	  7.2%
Trp (W)   4	  1.8%
Tyr (Y)   3	  1.3%
Val (V)  19	  8.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 27

Atomic composition:

Carbon      C	      1105
Hydrogen    H	      1789
Nitrogen    N	       315
Oxygen      O	       333
Sulfur      S	        11

Formula: C1105H1789N315O333S11
Total number of atoms: 3553

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26595
Abs 0.1% (=1 g/l)   1.057, assuming all pairs of Cys residues form cystines


Ext. coefficient    26470
Abs 0.1% (=1 g/l)   1.052, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.93
This classifies the protein as stable.



Aliphatic index: 94.35

Grand average of hydropathicity (GRAVY): -0.175