ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKSCRSCKEN PEVQRKRAEK PWRRAPKAKS EVEKHGIGPP DSDSGEEISF PWYNVTGKML 

        70         80         90        100        110        120 
VVGMHVEIKR SDGRIHGAVI AEVKSNGRFM VEWYEKGETK GKESSLEELL TLNPSLQAPK 

       130        140        150        160        170        180 
PTPPPQPPQK TLQASTAVNR QNGIHAQSMI LDDEDTFLLD HINMIPAKGA SANKRPTGAF 

       190        200        210        220        230        240 
TQHVPVRMAP PSEKPLPTRR APSPKEDAAP APKSTRTTAA FKPDLDSTAI TVPKHTARRT 

       250        260        270        280        290        300 
VVVAPAPAPV PAPPIANMTS QRAPSPVARV PSPKNVPRSY PQQDVQPSNG NFAFAEMIRN 

       310        320        330        340        350        360 
YRAQIDYRPL SMFDGVNENR ISVCVRKRPL NKKELTKAEV DVITIPSRDI TILHQPQTRV 

       370        380        390        400        410        420 
DLTKYLDNQK FRFDYSFDEY ANNELVYRFT AAPLVKTVFD NGTATCFAYG QTGSGKTHTM 

       430        440        450        460        470        480 
GGDFSGKKQN ASMGIYALTA RDVFRMLEQP QYRRKDLSVH CAFFEIYGTK TYDLLNDKAE 

       490        500        510        520        530        540 
LRVLEDKMQK VQVVGLKEEQ AHNEQDVLEL INKGTLVRTA GTTSANANSS RSHAIFQIIL 

       550        560        570        580        590        600 
RQGKKVWGKF SLIDLAGNER GQDTRECDRD TRKEGANINT SLLALKECIR GMARNSSHVP 

       610        620        630        640        650        660 
FRQSKLTMVL RDSFIGEKSR TVMISMISPG ISSSDHTLNT LRYADRVKEM GTDGSGEATP 

       670        680        690        700        710        720 
IRDEELFLPP SADKSDEEYD EMVEKQEHRR VAVDHVRNLK DMSEKIIRET TIVLSNEPSA 

       730        740 
AQKAECLAKL DQLAQIVSNT RVAVENV 

References and documentation are available.

Number of amino acids: 747

Molecular weight: 83342.70

Theoretical pI: 9.18

Amino acid composition: 
Ala (A)  62	  8.3%
Arg (R)  54	  7.2%
Asn (N)  35	  4.7%
Asp (D)  42	  5.6%
Cys (C)   8	  1.1%
Gln (Q)  32	  4.3%
Glu (E)  51	  6.8%
Gly (G)  38	  5.1%
His (H)  16	  2.1%
Ile (I)  36	  4.8%
Leu (L)  48	  6.4%
Lys (K)  54	  7.2%
Met (M)  21	  2.8%
Phe (F)  23	  3.1%
Pro (P)  52	  7.0%
Ser (S)  54	  7.2%
Thr (T)  49	  6.6%
Trp (W)   4	  0.5%
Tyr (Y)  16	  2.1%
Val (V)  52	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 93
Total number of positively charged residues (Arg + Lys): 108

Atomic composition:

Carbon      C	      3635
Hydrogen    H	      5856
Nitrogen    N	      1066
Oxygen      O	      1120
Sulfur      S	        29

Formula: C3635H5856N1066O1120S29
Total number of atoms: 11706

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    46340
Abs 0.1% (=1 g/l)   0.556, assuming all pairs of Cys residues form cystines


Ext. coefficient    45840
Abs 0.1% (=1 g/l)   0.550, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.58
This classifies the protein as unstable.



Aliphatic index: 72.34

Grand average of hydropathicity (GRAVY): -0.624