ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKSELNLPAG FRFHPTDEEL VKFYLCRKCA SEQISAPVIA EIDLYKFNPW ELPEMSLYGE 

        70         80         90        100        110        120 
KEWYFFSPRD RKYPNGSRPN RAAGTGYWKA TGADKPIGKP KTLGIKKALV FYAGKAPKGI 

       130        140        150        160        170        180 
KTNWIMHEYR LANVDRSASV NKKNNLRLDD WVLCRIYNKK GTMEKYFPAD EKPRTTTMAE 

       190        200        210        220        230        240 
QSSSPFDTSD STYPTLQEDD SSSSGGHGHV VSPDVLEVQS EPKWGELEDA LEAFDTSMFG 

       250        260        270        280 
SSMELLQPDA FVPQFLYQSD YFTSFQDPPE QKPFLNWSFA PQG 

References and documentation are available.

Number of amino acids: 283

Molecular weight: 32225.23

Theoretical pI: 5.55

Amino acid composition: 
Ala (A)  19	  6.7%
Arg (R)  11	  3.9%
Asn (N)  11	  3.9%
Asp (D)  18	  6.4%
Cys (C)   3	  1.1%
Gln (Q)  10	  3.5%
Glu (E)  21	  7.4%
Gly (G)  17	  6.0%
His (H)   4	  1.4%
Ile (I)   8	  2.8%
Leu (L)  21	  7.4%
Lys (K)  23	  8.1%
Met (M)   7	  2.5%
Phe (F)  17	  6.0%
Pro (P)  23	  8.1%
Ser (S)  25	  8.8%
Thr (T)  14	  4.9%
Trp (W)   7	  2.5%
Tyr (Y)  13	  4.6%
Val (V)  11	  3.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1456
Hydrogen    H	      2187
Nitrogen    N	       375
Oxygen      O	       435
Sulfur      S	        10

Formula: C1456H2187N375O435S10
Total number of atoms: 4463

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    57995
Abs 0.1% (=1 g/l)   1.800, assuming all pairs of Cys residues form cystines


Ext. coefficient    57870
Abs 0.1% (=1 g/l)   1.796, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.88
This classifies the protein as unstable.



Aliphatic index: 57.95

Grand average of hydropathicity (GRAVY): -0.686