ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKSGSGGGSP TSLWGLVFLS AALSLWPTSG EICGPGIDIR NDYQQLKRLE NCTVIEGFLH 

        70         80         90        100        110        120 
ILLISKAEDY RSYRFPKLTV ITEYLLLFRV AGLESLGDLF PNLTVIRGWK LFYNYALVIF 

       130        140        150        160        170        180 
EMTNLKDIGL YNLRNITRGA IRIEKNADLC YLSTIDWSLI LDAVSNNYIV GNKPPKECGD 

       190        200        210        220        230        240 
LCPGTLEEKP MCEKTTINNE YNYRCWTTNR CQKMCPSVCG KRACTENNEC CHPECLGSCH 

       250        260        270        280        290        300 
TPDDNTTCVA CRHYYYKGVC VPACPPGTYR FEGWRCVDRD FCANIPNAES SDSDGFVIHD 

       310        320        330        340        350        360 
GECMQECPSG FIRNSTQSMY CIPCEGPCPK VCGDEEKKTK TIDSVTSAQM LQGCTILKGN 

       370        380        390        400        410        420 
LLINIRRGNN IASELENFMG LIEVVTGYVK IRHSHALVSL SFLKNLRLIL GEEQLEGNYS 

       430        440        450        460        470        480 
FYVLDNQNLQ QLWDWNHRNL TVRSGKMYFA FNPKLCVSEI YRMEEVTGTK GRQSKGDINT 

       490        500        510        520        530        540 
RNNGERASCE SDVLRFTSTT TWKNRIIITW HRYRPPDYRD LISFTVYYKE APFKNVTEYD 

       550        560        570        580        590        600 
GQDACGSNSW NMVDVDLPPN KEGEPGILLH GLKPWTQYAV YVKAVTLTMV ENDHIRGAKS 

       610        620        630        640        650        660 
EILYIRTNAS VPSIPLDVLS ASNSSSQLIV KWNPPTLPNG NLSYYIVRWQ RQPQDGYLFR 

       670        680        690        700        710        720 
HNYCSKDKIP IRKYADGTID VEEVTENPKT EVCGGDKGPC CACPKTEAEK QAEKEEAEYR 

       730        740        750        760        770        780 
KVFENFLHNS IFVPRPERRR RDVLQVANTT MSSRSRNTTV ADTYNITDPE EFETEYPFFE 

       790        800        810        820        830        840 
SRVDNKERTV ISNLRPFTLY RIDIHSCNHE AEKLGCSASN FVFARTMPAE GADDIPGPVT 

       850        860        870        880        890        900 
WEPRPENSIF LKWPEPENPN GLILMYEIKY GSQVEDQREC VSRQEYRKYG GAKLNRLNPG 

       910        920        930        940        950        960 
NYTARIQATS LSGNGSWTDP VFFYVPAKTT YENFMHLIIA LPVAILLIVG GLVIMLYVFH 

       970        980        990       1000       1010       1020 
RKRNNSRLGN GVLYASVNPE YFSAADVYVP DEWEVAREKI TMNRELGQGS FGMVYEGVAK 

      1030       1040       1050       1060       1070       1080 
GVVKDEPETR VAIKTVNEAA SMRERIEFLN EASVMKEFNC HHVVRLLGVV SQGQPTLVIM 

      1090       1100       1110       1120       1130       1140 
ELMTRGDLKS YLRSLRPEVE NNLVLIPPSL SKMIQMAGEI ADGMAYLNAN KFVHRDLAAR 

      1150       1160       1170       1180       1190       1200 
NCMVAEDFTV KIGDFGMTRD IYETDYYRKG GKGLLPVRWM SPESLKDGVF TTHSDVWSFG 

      1210       1220       1230       1240       1250       1260 
VVLWEIATLA EQPYQGLSNE QVLRFVMEGG LLDKPDNCPD MLFELMRMCW QYNPKMRPSF 

      1270       1280       1290       1300       1310       1320 
LEIIGSIKDE MEPSFQEVSF YYSEENKPPE PEELEMELEL EPENMESVPL DPSASSASLP 

      1330       1340       1350       1360       1370 
LPERHSGHKA ENGPGVLVLR ASFDERQPYA HMNGGRANER ALPLPQSSTC 

References and documentation are available.

Number of amino acids: 1370

Molecular weight: 155395.77

Theoretical pI: 5.57

Amino acid composition: 
Ala (A)  68	  5.0%
Arg (R)  82	  6.0%
Asn (N)  87	  6.4%
Asp (D)  62	  4.5%
Cys (C)  44	  3.2%
Gln (Q)  35	  2.6%
Glu (E) 114	  8.3%
Gly (G)  91	  6.6%
His (H)  24	  1.8%
Ile (I)  73	  5.3%
Leu (L) 117	  8.5%
Lys (K)  67	  4.9%
Met (M)  38	  2.8%
Phe (F)  49	  3.6%
Pro (P)  82	  6.0%
Ser (S)  92	  6.7%
Thr (T)  73	  5.3%
Trp (W)  22	  1.6%
Tyr (Y)  60	  4.4%
Val (V)  90	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 176
Total number of positively charged residues (Arg + Lys): 149

Atomic composition:

Carbon      C	      6878
Hydrogen    H	     10689
Nitrogen    N	      1875
Oxygen      O	      2070
Sulfur      S	        82

Formula: C6878H10689N1875O2070S82
Total number of atoms: 21594

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   213150
Abs 0.1% (=1 g/l)   1.372, assuming all pairs of Cys residues form cystines


Ext. coefficient   210400
Abs 0.1% (=1 g/l)   1.354, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.27
This classifies the protein as unstable.



Aliphatic index: 78.10

Grand average of hydropathicity (GRAVY): -0.399