ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKSLNDLPAP KSTTTTYYDH SNDAWFKNRV TESETVKSSS IKFKVVPAYL NRQGLRPKNP 

        70         80         90        100        110        120 
EDFGDGGAFP EIHLPQYPLL MGKNKSNKPG AKTLPVTVDA QGNVVFDAIV RQNENSRKIV 

       130        140        150        160        170        180 
YSQHKDIIPK FLKNEGDLGT VVDEEEELQK EIQETAEETK AAIEKIVNVR LSAAQPSNIA 

       190        200        210        220        230        240 
RQSGDSQYIK YKPSQQSSAF NSGAKERIIR MVEMPVDPLD PPKFKHKRVP RASGSPPVPV 

       250        260        270        280        290        300 
MHSPPRPVTV KDQQDWKIPP CISNWKNPKG YTIPLDKRLA ADGRGLQDVQ INDNFAKLSE 

       310        320        330        340        350        360 
ALYVAEQKAR EAVSMRSKVQ KEMVMKDKER KEQELRALAQ KARSERTGAA MSMPVSSDRG 

       370        380        390        400        410        420 
RSESVDPRGD YDNYDQDRGR EREREEPQET REEREKRIQR EKIREERRRE RERERRLDAK 

       430        440        450        460        470        480 
DAAMGKKSKI TRDRDRDISE KVALGMASTG GKGGGEVMYD QRLFNQDKGM DSGFAADDQY 

       490        500        510        520        530        540 
NLYDKGLFTA QPTLSTLYKP KKDNDEEMYG NADEQLDKIK NTERFKPDKA FTGASERVGS 

       550        560        570        580        590        600 
KRDRPVEFEK EEEQDPFGLE KWVSDLKKGK KPLDKIGSGG TMRASGGGGS SSRDDDHGGS 

       610 
GRTKINFERS DRR 

References and documentation are available.

Number of amino acids: 613

Molecular weight: 69422.73

Theoretical pI: 9.18

Amino acid composition: 
Ala (A)  40	  6.5%
Arg (R)  53	  8.6%
Asn (N)  25	  4.1%
Asp (D)  50	  8.2%
Cys (C)   1	  0.2%
Gln (Q)  29	  4.7%
Glu (E)  54	  8.8%
Gly (G)  44	  7.2%
His (H)   6	  1.0%
Ile (I)  24	  3.9%
Leu (L)  31	  5.1%
Lys (K)  63	 10.3%
Met (M)  16	  2.6%
Phe (F)  17	  2.8%
Pro (P)  37	  6.0%
Ser (S)  46	  7.5%
Thr (T)  24	  3.9%
Trp (W)   4	  0.7%
Tyr (Y)  16	  2.6%
Val (V)  33	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 104
Total number of positively charged residues (Arg + Lys): 116

Atomic composition:

Carbon      C	      2993
Hydrogen    H	      4823
Nitrogen    N	       905
Oxygen      O	       962
Sulfur      S	        17

Formula: C2993H4823N905O962S17
Total number of atoms: 9700

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    45840
Abs 0.1% (=1 g/l)   0.660, assuming all pairs of Cys residues form cystines


Ext. coefficient    45840
Abs 0.1% (=1 g/l)   0.660, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.25
This classifies the protein as unstable.



Aliphatic index: 57.13

Grand average of hydropathicity (GRAVY): -1.133