ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKSTRCLPVS WPVAEKFWWD LAMEWKGLPS ACEAKTASFQ DAVRTLNTLQ TNASCLEEVK 

        70         80         90        100        110        120 
RQRSDPQAQL EAMEVYLARS GLQVEDLNQL KIIHVTGTKG KGSTCAFTER ILRNYGLKTG 

       130        140        150        160        170        180 
FFSSPHLVQV RERIRINGKP ISPELFTKYF WHLYQQLEEF KDDSHISMPS YFRFLTLMAF 

       190        200        210        220        230        240 
HVFLQEKVDL AVVEVGIGGA FDCTNIIRKP VVCGVSSLGI DHTSLLGDTV EKIAWQKGGI 

       250        260        270        280        290        300 
FKPGVPAFTV LQPEGPLAVL RDRAQQISCP LYLCPPLEAL EQVGLPLSLG LEGEHQRHNA 

       310        320        330        340        350        360 
ALALQLARCW LEQQNYHDIQ ELKASKPSIR WQLPLAPVFR PTPHMRRGLR DTEWPGRTQV 

       370        380        390        400        410        420 
LQRGPVTWYL DGAHTTSSVQ ACVHWYCQSL ERSRRTDGGH EVHILLFNST GDRDSAALLK 

       430        440        450        460        470        480 
LLQPCHFDYA VFCPNVTEIS SIGNADQQNF TVTLDQVLLR CLQHQKHWNC LAEKQASSNL 

       490        500        510        520        530        540 
WGSCGPESAR PGSLLLAPHP PQPTSTSSLV FSCISHALQW ISQGRDPIFQ PESPPRNLLN 

       550        560        570        580 
HPTANSGASI LREAAAIHVL VTGSLHLVGG ALRLLDPSMS Q 

References and documentation are available.

Number of amino acids: 581

Molecular weight: 64859.33

Theoretical pI: 7.87

Amino acid composition: 
Ala (A)  42	  7.2%
Arg (R)  33	  5.7%
Asn (N)  17	  2.9%
Asp (D)  22	  3.8%
Cys (C)  17	  2.9%
Gln (Q)  38	  6.5%
Glu (E)  31	  5.3%
Gly (G)  37	  6.4%
His (H)  22	  3.8%
Ile (I)  24	  4.1%
Leu (L)  72	 12.4%
Lys (K)  22	  3.8%
Met (M)   7	  1.2%
Phe (F)  22	  3.8%
Pro (P)  38	  6.5%
Ser (S)  46	  7.9%
Thr (T)  31	  5.3%
Trp (W)  14	  2.4%
Tyr (Y)  10	  1.7%
Val (V)  36	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2899
Hydrogen    H	      4539
Nitrogen    N	       815
Oxygen      O	       830
Sulfur      S	        24

Formula: C2899H4539N815O830S24
Total number of atoms: 9107

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    92900
Abs 0.1% (=1 g/l)   1.432, assuming all pairs of Cys residues form cystines


Ext. coefficient    91900
Abs 0.1% (=1 g/l)   1.417, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.71
This classifies the protein as unstable.



Aliphatic index: 89.64

Grand average of hydropathicity (GRAVY): -0.201