ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKSYNVLLLA SLFLSLMLLC PVWAQFPREC ANIEALRSGV CCPDLFPSSG PGTDPCGSSS 

        70         80         90        100        110        120 
GRGRCVAVTA DSRPHSRHYP HDGRDDREGW PLRFFNRTCQ CNGNFSGHNC GACRPGWRGA 

       130        140        150        160        170        180 
ACNQKILTVI RRNLLDLSTE EKSHFVRALD MAKRTTHPQF VIATRRSEDM LGPDGSTPQF 

       190        200        210        220        230        240 
ENISIYNYFV WTHYYSVKKT FLGAGQESFG DVDFSHEGPA FLTWHRYHLL QLERDIQEML 

       250        260        270        280        290        300 
QEPSFSLPYW NFATGKNICD ICTDDLMGSR SNFDSTLISP NSVFSQWRVV CESLEEYDTL 

       310        320        330        340        350        360 
GTLCNSTEGG PIRRNPAGNV GRPAVQRLPA PQDVTRCLEV RVFDTPPFYS NSTNSFRNTV 

       370        380        390        400        410        420 
EGYSDPTGKY DPAVRSLHNL AHLFLNGTGG QTHLSPNDPI FVLLHTFTDA VFDEWLRRYN 

       430        440        450        460        470        480 
ADISTFPLEN APIGHNRQYN MVPFWPPVTN TEMFVTAPDN LGYAYELQWP GQEFTVSEII 

       490        500        510        520        530 
TIAVIAGLLL VAAIFGSASC LIRSRSTKNE ANQPLLTDHY QRYAEEYEEL PNPNQTMV 

References and documentation are available.

Number of amino acids: 538

Molecular weight: 60565.06

Theoretical pI: 5.75

Amino acid composition: 
Ala (A)  32	  5.9%
Arg (R)  36	  6.7%
Asn (N)  33	  6.1%
Asp (D)  28	  5.2%
Cys (C)  17	  3.2%
Gln (Q)  19	  3.5%
Glu (E)  29	  5.4%
Gly (G)  36	  6.7%
His (H)  16	  3.0%
Ile (I)  20	  3.7%
Leu (L)  49	  9.1%
Lys (K)   9	  1.7%
Met (M)   9	  1.7%
Phe (F)  30	  5.6%
Pro (P)  38	  7.1%
Ser (S)  42	  7.8%
Thr (T)  36	  6.7%
Trp (W)  10	  1.9%
Tyr (Y)  19	  3.5%
Val (V)  30	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      2689
Hydrogen    H	      4079
Nitrogen    N	       749
Oxygen      O	       802
Sulfur      S	        26

Formula: C2689H4079N749O802S26
Total number of atoms: 8345

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    84310
Abs 0.1% (=1 g/l)   1.392, assuming all pairs of Cys residues form cystines


Ext. coefficient    83310
Abs 0.1% (=1 g/l)   1.376, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 54.91
This classifies the protein as unstable.



Aliphatic index: 72.14

Grand average of hydropathicity (GRAVY): -0.361