ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKTLETEKWK ILGTLKPENF AEMVTKMKDD EVYVDPHVEN LIIFAPNIQN FYEYWMVEQR 

        70         80         90        100        110        120 
YLILQDKASC LPAFLLNPRP GSQVFDTCAA PGMKTSHAAA IMENQGKVWA MDRAADRVAT 

       130        140        150        160        170        180 
MKQLLDASKV AIASSFCGDF LKTDVTDKKF SKVKFAIVDP PCSGSGIVKR MDEITGGNAE 

       190        200        210        220        230        240 
KERLEKLKNL QAMILKHALK LPGLKRAVYS TCSVHEEENE QVVDEVLLDT YVRQNYVLKK 

       250        260        270        280 
NVLPEWTYRG LKTYEVGEHC LRANPKVTLT NGFFVAVFER VKSSE 

References and documentation are available.

Number of amino acids: 285

Molecular weight: 32309.42

Theoretical pI: 7.57

Amino acid composition: 
Ala (A)  23	  8.1%
Arg (R)  11	  3.9%
Asn (N)  13	  4.6%
Asp (D)  15	  5.3%
Cys (C)   6	  2.1%
Gln (Q)   9	  3.2%
Glu (E)  23	  8.1%
Gly (G)  13	  4.6%
His (H)   5	  1.8%
Ile (I)  11	  3.9%
Leu (L)  26	  9.1%
Lys (K)  28	  9.8%
Met (M)  10	  3.5%
Phe (F)  12	  4.2%
Pro (P)  12	  4.2%
Ser (S)  13	  4.6%
Thr (T)  16	  5.6%
Trp (W)   4	  1.4%
Tyr (Y)   9	  3.2%
Val (V)  26	  9.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 39

Atomic composition:

Carbon      C	      1447
Hydrogen    H	      2296
Nitrogen    N	       382
Oxygen      O	       422
Sulfur      S	        16

Formula: C1447H2296N382O422S16
Total number of atoms: 4563

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    35785
Abs 0.1% (=1 g/l)   1.108, assuming all pairs of Cys residues form cystines


Ext. coefficient    35410
Abs 0.1% (=1 g/l)   1.096, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 20.81
This classifies the protein as stable.



Aliphatic index: 85.16

Grand average of hydropathicity (GRAVY): -0.279