ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKTLIVVDMQ NDFISPLGSL TVPKGEELIN PISDLMQDAD RDWHRIVVTR DWHPSRHISF 

        70         80         90        100        110        120 
AKNHKDKEPY STYTYHSPRP GDDSTQEGIL WPVHCVKNTW GSQLVDQIMD QVVTKHIKIV 

       130        140        150        160        170        180 
DKGFLTDREY YSAFHDIWNF HKTDMNKYLE KHHTDEVYIV GVALEYCVKA TAISAAELGY 

       190        200        210 
KTTVLLDYTR PISDDPEVIN KVKEELKAHN INVVDK 

References and documentation are available.

Number of amino acids: 216

Molecular weight: 24993.31

Theoretical pI: 5.81

Amino acid composition: 
Ala (A)   9	  4.2%
Arg (R)   7	  3.2%
Asn (N)   9	  4.2%
Asp (D)  21	  9.7%
Cys (C)   2	  0.9%
Gln (Q)   6	  2.8%
Glu (E)  12	  5.6%
Gly (G)   8	  3.7%
His (H)  12	  5.6%
Ile (I)  16	  7.4%
Leu (L)  14	  6.5%
Lys (K)  18	  8.3%
Met (M)   5	  2.3%
Phe (F)   5	  2.3%
Pro (P)  10	  4.6%
Ser (S)  12	  5.6%
Thr (T)  15	  6.9%
Trp (W)   5	  2.3%
Tyr (Y)  10	  4.6%
Val (V)  20	  9.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 25

Atomic composition:

Carbon      C	      1122
Hydrogen    H	      1731
Nitrogen    N	       299
Oxygen      O	       335
Sulfur      S	         7

Formula: C1122H1731N299O335S7
Total number of atoms: 3494

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42525
Abs 0.1% (=1 g/l)   1.701, assuming all pairs of Cys residues form cystines


Ext. coefficient    42400
Abs 0.1% (=1 g/l)   1.696, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 27.12
This classifies the protein as stable.



Aliphatic index: 85.19

Grand average of hydropathicity (GRAVY): -0.514