ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKTLVELPLL PLRDVVVYPH MVIPLFVGRE KSIEALEAAM TGDKQILLLA QKNPADDDPG 

        70         80         90        100        110        120 
EDGLYRMGTV ATVLQLLKLP DGTVKVLVEG EQRGQVERFI EEEGHIRAAV QAIDDANVGE 

       130        140        150        160        170        180 
REAEVFTRSL LSQFEQYVQL GKKVPAEVLS SLNSIDEPSR LVDTMAAHMA LKIEQKQDIL 

       190        200        210        220        230        240 
EITDLSSRVE HVLALLDAEI DLLQVEKRIR GRVKKQMERS QREYYLNEQM KAIQKELGDI 

       250        260        270        280        290        300 
DEGHNEVEEL KKRIDAAGLT KEAHTKATAE LNKLKQMSPM SAEATVVRSY IDWLLNVPWK 

       310        320        330        340        350        360 
AESKVRHDLA KAEDILDADH YGLEEVKERI LEYLAVQKRV KKLKGPVLCL VGPPGVGKTS 

       370        380        390        400        410        420 
LAESIARATN RKFVRMALGG VRDEAEIRGH RRTYIGSMPG RLIQKMTKVG VRNPLFLLDE 

       430        440        450        460        470        480 
IDKMGSDMRG DPASALLEVL DPEQNHNFND HYLEVDYDLS DVMFLCTANS MNIPAPLLDR 

       490        500        510        520        530        540 
MEVIRLPGYT EDEKVNIAAK YLIPKQVQAN GLKKGELTFE EGALRDIIRY YTREAGVRSL 

       550        560        570        580        590        600 
ERQIAKVCRK AVKEHAKLKR IQAVVSSETL ENYLGVRKFR YGLAEQQDQI GQVTGLAWTQ 

       610        620        630        640        650        660 
VGGELLTIEA AVVPGKGQLT KTGSLGDVMA ESITAALTVV RSRAQSLGIA ADFHEKRDIH 

       670        680        690        700        710        720 
IHVPEGATPK DGPSAGIGMC TALVSAITQI PVRADVAMTG EITLRGQVLA IGGLKEKLLA 

       730        740        750        760        770        780 
AHRGGIKTVI IPEENVRDLK EIPDNIKSDL VIKPVKWIDE VLQIALQYAP EPLPDAAPEM 

       790 
VAKDEKREPD SKERISTH 

References and documentation are available.

Number of amino acids: 798

Molecular weight: 88647.24

Theoretical pI: 5.81

Amino acid composition: 
Ala (A)  72	  9.0%
Arg (R)  50	  6.3%
Asn (N)  19	  2.4%
Asp (D)  49	  6.1%
Cys (C)   4	  0.5%
Gln (Q)  35	  4.4%
Glu (E)  74	  9.3%
Gly (G)  54	  6.8%
His (H)  17	  2.1%
Ile (I)  51	  6.4%
Leu (L)  88	 11.0%
Lys (K)  59	  7.4%
Met (M)  22	  2.8%
Phe (F)  11	  1.4%
Pro (P)  35	  4.4%
Ser (S)  33	  4.1%
Thr (T)  35	  4.4%
Trp (W)   4	  0.5%
Tyr (Y)  18	  2.3%
Val (V)  68	  8.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 123
Total number of positively charged residues (Arg + Lys): 109

Atomic composition:

Carbon      C	      3912
Hydrogen    H	      6423
Nitrogen    N	      1099
Oxygen      O	      1185
Sulfur      S	        26

Formula: C3912H6423N1099O1185S26
Total number of atoms: 12645

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49070
Abs 0.1% (=1 g/l)   0.554, assuming all pairs of Cys residues form cystines


Ext. coefficient    48820
Abs 0.1% (=1 g/l)   0.551, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.70
This classifies the protein as unstable.



Aliphatic index: 101.67

Grand average of hydropathicity (GRAVY): -0.279