ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKTQYSLIRK IWAHSVTEFL KSQWFFICLA ILIVIARFAP NFARDGGLIK GQYSIGYGCV 

        70         80         90        100        110        120 
AWIFLQSGLG MKSRSLMANM LNWRAHATIL VLSFLITSSI VYGFCCAVKA ANDPKIDDWV 

       130        140        150        160        170        180 
LIGLILTATC PTTVASNVIM TTNAGGNSLL CVCEVFIGNL LGAFITPALV QMFTNRAPFA 

       190        200        210        220        230        240 
YGNPATGNGI GALYGRVMKQ VGLSVFVPLF VGQVIQNCFP KGTAYYLGFL KKYHIKIGSY 

       250        260        270        280        290        300 
MLLLIMFSSF STAFYQDAFT SVSHVCIIFL CFFNLGIYIF FTGLSYLCAR PWFILKLFPH 

       310        320        330        340        350        360 
EPIEGKSTRL YRYSYNIFRP FYYSKEDAIC IMFCGPAKTA ALGVSLITSQ YGDKKEHLGK 

       370        380        390        400        410        420 
LLVPLVLYQV EQVMTANFFV SLFKRWIQKD AQADGSESSC ANENEEVDLE KIISIGTGEN 

       430 
QSVLSNNVPY TQPR 

References and documentation are available.

Number of amino acids: 434

Molecular weight: 48374.78

Theoretical pI: 9.01

Amino acid composition: 
Ala (A)  33	  7.6%
Arg (R)  13	  3.0%
Asn (N)  20	  4.6%
Asp (D)  10	  2.3%
Cys (C)  14	  3.2%
Gln (Q)  16	  3.7%
Glu (E)  13	  3.0%
Gly (G)  33	  7.6%
His (H)   6	  1.4%
Ile (I)  37	  8.5%
Leu (L)  45	 10.4%
Lys (K)  22	  5.1%
Met (M)  11	  2.5%
Phe (F)  33	  7.6%
Pro (P)  16	  3.7%
Ser (S)  32	  7.4%
Thr (T)  23	  5.3%
Trp (W)   7	  1.6%
Tyr (Y)  21	  4.8%
Val (V)  29	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      2241
Hydrogen    H	      3438
Nitrogen    N	       550
Oxygen      O	       593
Sulfur      S	        25

Formula: C2241H3438N550O593S25
Total number of atoms: 6847

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    70665
Abs 0.1% (=1 g/l)   1.461, assuming all pairs of Cys residues form cystines


Ext. coefficient    69790
Abs 0.1% (=1 g/l)   1.443, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 24.68
This classifies the protein as stable.



Aliphatic index: 100.67

Grand average of hydropathicity (GRAVY): 0.421