ProtParam
User-provided sequence:
10 20 30 40 50 60
MKTTMLMLVL LVCSYIHYVC AQIRFVTPAT TDSMDFTAIS FSWEESNTGI PLEDITNTVF
70 80 90 100 110 120
YICSGSMDAP QPCAVLYTSP SPSSISQAGP FAISQVFGPA GRLYFLWAQS TYAGGIVNDY
130 140 150 160 170 180
TDFFTVNGLT GTFDNYEIYA SLMALGVYPY VPTLTGFSTF LGVWPTGTMR DWYLSQTTGV
190 200 210 220 230 240
LRTGPIQNRP DSTFTAATTD IQPLWETSSY SVFTTFAGPP IATSTVFASP TYMYTLYANY
250 260 270 280
ASTASKPTII ATPTAGLRRR DSWAQAAPKR GMRLGEHKRG LLYS
References and
documentation are available.
Number of amino acids: 284
Molecular weight: 31132.36
Theoretical pI: 5.63
Total number of negatively charged residues (Asp + Glu): 17
Total number of positively charged residues (Arg + Lys): 15
Atomic composition:
Carbon C 1417
Hydrogen H 2131
Nitrogen N 347
Oxygen O 418
Sulfur S 13
Formula: C
1417H
2131N
347O
418S
13
Total number of atoms: 4326
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 60070
Abs 0.1% (=1 g/l) 1.930, assuming all pairs of Cys residues form cystines
Ext. coefficient 59820
Abs 0.1% (=1 g/l) 1.921, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.00
This classifies the protein as unstable.
Aliphatic index: 73.56
Grand average of hydropathicity (GRAVY): 0.082