ProtParam
User-provided sequence:
10 20 30 40 50 60
MKTVAALTLL AFATAANGQF LQDCTNALDG LYALGECEPQ FLTCSGGIAR IMDCPADLIY
70 80 90 100 110 120
NEPLLICDWR HNVIGCEGSG ESSGETSGEG SGESSGEASG EGSGEASGEG SGEASGEGSG
130 140 150 160 170 180
EASGEGSGSG EETVENVCEN LEDGAYSSGG CTTYYFFCTT NTARFLSCPT PLFYDADSQK
190 200 210 220 230 240
CIWKSLVEEC KEDLTITDGS GETSGEGSGE ASGEASGEGS GEASGESSGQ GSGEASGEGS
250 260 270 280 290 300
GELEPTCEGK ADGIHPNGVC STNFLTCSGG IARIMDCPAS LVFNPTILVC DWPRDVAECA
310 320 330 340 350 360
GLPTPQPTCE EDGYFSFGQC SSSFTACTNG RAIVMFCPAG LKFSESTVRC DYESNVSECQ
370 380 390 400 410 420
ETSGEESGEA SGEQSGEGSG EASGEASGES SGEGSGVEEQ NQCVGLDNGL HAIGCSPRVL
430 440 450 460 470 480
SCQNGHVDIF ECPSSLVFND QSLICDYPQT SLKCLIEDTI LIDETPIAAF DCSTDGLFSD
490 500 510 520
GLCSATYHQC TAGQLINFTC AASNAVFSAA NTECVDSSTL LQCH
References and
documentation are available.
Number of amino acids: 524
Molecular weight: 53651.96
Theoretical pI: 3.84
Total number of negatively charged residues (Asp + Glu): 84
Total number of positively charged residues (Arg + Lys): 15
Atomic composition:
Carbon C 2243
Hydrogen H 3442
Nitrogen N 606
Oxygen O 842
Sulfur S 40
Formula: C
2243H
3442N
606O
842S
40
Total number of atoms: 7173
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 33650
Abs 0.1% (=1 g/l) 0.627, assuming all pairs of Cys residues form cystines
Ext. coefficient 31400
Abs 0.1% (=1 g/l) 0.585, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.44
This classifies the protein as stable.
Aliphatic index: 59.47
Grand average of hydropathicity (GRAVY): -0.235