ProtParam
User-provided sequence:
10 20 30 40 50 60
MKTYHLNNDI IVTQEQLDHW NEQLIKLETP QEIIAWSIVT FPHLFQTTAF GLTGLVTIDM
70 80 90 100 110 120
LSKLSEKYYM PELLFIDTLH HFPQTLTLKN EIEKKYYQPK NQTIHVYKPD GCESEADFAS
130 140 150 160 170 180
KYGDFLWEKD DDKYDYLAKV EPAHRAYKEL HISAVFTGRR KSQGSARSQL SIIEIDELNG
190 200 210 220 230 240
ILKINPLINW TFEQVKQYID ANNVPYNELL DLGYRSIGDY HSTQPVKEGE DERAGRWKGK
250 260
AKTECGIHEA SRFAQFLKQD A
References and
documentation are available.
Number of amino acids: 261
Molecular weight: 30380.32
Theoretical pI: 5.59
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 30
Atomic composition:
Carbon C 1377
Hydrogen H 2106
Nitrogen N 358
Oxygen O 409
Sulfur S 5
Formula: C
1377H
2106N
358O
409S
5
Total number of atoms: 4255
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 48485
Abs 0.1% (=1 g/l) 1.596, assuming all pairs of Cys residues form cystines
Ext. coefficient 48360
Abs 0.1% (=1 g/l) 1.592, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.15
This classifies the protein as unstable.
Aliphatic index: 82.99
Grand average of hydropathicity (GRAVY): -0.596