ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKVEEHVPLI QESRKRKCQS SENASKRQQL LSKLPLRLHT GNENVDLSPL VSAIRKAKRI 

        70         80         90        100        110        120 
VVVTGAGISC DAGIPDFRSS EGLFSSLRAE YKLNCSGKEL FDGSVYRDLK SVNIFHAMIR 

       130        140        150        160        170        180 
KLHMLSNNAR PTDFHLFLSQ LAQESKLLRL YTQNIDFLET RLEGLQTCIP LPQSAPWPTT 

       190        200        210        220        230        240 
IPLHGTLEVV SCTRCSFLKK FNPDIFDRNG VTVCPDCKTE NEVRRIAGKR SVIEGCLRPR 

       250        260        270        280        290        300 
IVLYNEIHPD SESIGSVCSQ DLKSRPDCLI VAGTSCKIPG VKRIIKEMSN CVHKQKGNVI 

       310        320        330        340        350        360 
WLNYDEPTKD FLNLCDLVVQ GDLQIAIRRL KPLLDAPSWK LKSHSAKRTS KQKSSEQTKI 

       370        380        390        400        410 
TSSTKITKAI GLNTKSNDSS KKDNTSFQLH QVLNSIEIPK VEIKQEVEYA TPSPL 

References and documentation are available.

Number of amino acids: 415

Molecular weight: 46589.82

Theoretical pI: 9.32

Amino acid composition: 
Ala (A)  17	  4.1%
Arg (R)  25	  6.0%
Asn (N)  20	  4.8%
Asp (D)  20	  4.8%
Cys (C)  14	  3.4%
Gln (Q)  18	  4.3%
Glu (E)  24	  5.8%
Gly (G)  18	  4.3%
His (H)  10	  2.4%
Ile (I)  29	  7.0%
Leu (L)  45	 10.8%
Lys (K)  37	  8.9%
Met (M)   4	  1.0%
Phe (F)  12	  2.9%
Pro (P)  22	  5.3%
Ser (S)  43	 10.4%
Thr (T)  22	  5.3%
Trp (W)   3	  0.7%
Tyr (Y)   6	  1.4%
Val (V)  26	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 62

Atomic composition:

Carbon      C	      2047
Hydrogen    H	      3356
Nitrogen    N	       588
Oxygen      O	       613
Sulfur      S	        18

Formula: C2047H3356N588O613S18
Total number of atoms: 6622

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26315
Abs 0.1% (=1 g/l)   0.565, assuming all pairs of Cys residues form cystines


Ext. coefficient    25440
Abs 0.1% (=1 g/l)   0.546, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.01
This classifies the protein as unstable.



Aliphatic index: 91.81

Grand average of hydropathicity (GRAVY): -0.388