ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKVKINEVHS VFAWSWHIPS TSDEDAANND PIGNDEDEDV CGICRASYNG TCPSCKFPGD 

        70         80         90        100        110        120 
QCPLVIGLCH HNFHDHCIYR WLDTPTSKGL CPMCRQTFQL QKGLAINDAH VQKFVEIVSR 

       130        140        150        160 
RREEMIEEGV AEEFVDFDEP IRQNTDNPIG RQQVDTILDE DFLLR 

References and documentation are available.

Number of amino acids: 165

Molecular weight: 18865.10

Theoretical pI: 4.72

Amino acid composition: 
Ala (A)   7	  4.2%
Arg (R)   9	  5.5%
Asn (N)   9	  5.5%
Asp (D)  16	  9.7%
Cys (C)   9	  5.5%
Gln (Q)   8	  4.8%
Glu (E)  13	  7.9%
Gly (G)   9	  5.5%
His (H)   7	  4.2%
Ile (I)  12	  7.3%
Leu (L)   9	  5.5%
Lys (K)   6	  3.6%
Met (M)   3	  1.8%
Phe (F)   8	  4.8%
Pro (P)   9	  5.5%
Ser (S)   8	  4.8%
Thr (T)   7	  4.2%
Trp (W)   3	  1.8%
Tyr (Y)   2	  1.2%
Val (V)  11	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 15

Atomic composition:

Carbon      C	       819
Hydrogen    H	      1256
Nitrogen    N	       232
Oxygen      O	       258
Sulfur      S	        12

Formula: C819H1256N232O258S12
Total number of atoms: 2577

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    19980
Abs 0.1% (=1 g/l)   1.059, assuming all pairs of Cys residues form cystines


Ext. coefficient    19480
Abs 0.1% (=1 g/l)   1.033, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.38
This classifies the protein as unstable.



Aliphatic index: 73.21

Grand average of hydropathicity (GRAVY): -0.511