ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKVKKSTRSK VSTACVNCRK RKIKCTGKYP CTNCISYDCT CVFLKKHLPQ KEDSSQSLPT 

        70         80         90        100        110        120 
TAVAPPSSHA NVEASADVQH LDTAIKLDNQ YYFKLMNDLI QTPVSPSATH APDTSNNPTN 

       130        140        150        160        170        180 
DNNILFKDDS KYQNQLVTYQ NILTNLYALP PCDDTQLLID KTKSQLNNLI NSWNPEINYP 

       190        200        210        220        230        240 
KLSSFSPRPQ RSIETYLLTN KYRNKIHMTR FSFWTDQMVK SQSPDSFLAT TPLVDEVFGL 

       250        260        270        280        290        300 
FSPIQAFSLR GIGYLIKKNI ENTGSSMLID TKETIYLILR LFDLCYEHLI QGCISISNPL 

       310        320        330        340        350        360 
ENYLQKIKQT PTTTASASLP TSPAPLSNDL VISVIHQLPQ PFIQSITGFT TTQLIENLHD 

       370        380        390        400        410        420 
SFSMFRIVTQ MYAQHRKRFA EFLNQAFSLP HQEKSVLFSS FCSSEYLLST LCYAYYNVTL 

       430        440        450        460        470        480 
YHMLDINTLD YLEILVSLLE IQNEIDERFG FEKMLEVAVT CSTKMGLSRW EYYVGIDENT 

       490        500        510        520        530        540 
AERRRKIWWK IYSLEKRFLT DLGDLSLINE HQMNCLLPKD FRDMGFINHK EFLTKIGTSS 

       550        560        570        580        590        600 
LSPSSPKLKN LSLSRLIEYG ELAIAQIVGD FFSETLYNEK FTSLEVSVKP TIIRQKLLEK 

       610        620        630        640        650        660 
VFEDIESFRL KLAKIKLHTS RVFQVAHCKY PEYPKNDLIE AAKFVSYHKN TWFSILGAVN 

       670        680        690        700        710        720 
NLIARLSEDP EVITEQSMKY ANEMFQEWRE INQFLIQVDT DFIVWACLDF YELIFFVMAS 

       730        740        750        760        770        780 
KFYVEDPHIT LEDVINTLKV FKRITNIISF FNNNLDEKDY DCQTFREFSR SSSLVAISIR 

       790        800        810        820        830        840 
IIFLKYCYAE QIDRAEFIER LKEVEPGLSD LLREFFDTRS FIYRYMLKSV EKSGFHLIIR 

       850        860        870        880        890        900 
KMLESDYKFL YRDKLATGNI PDQGNSSQIS QLYDSTAPSY NNASASAANS PLKLSSLLNS 

       910        920        930        940        950        960 
GEESYTQDAS ENVPCNLRHQ DRSLQQTKRQ HSAPSQISAN ENNIYNLGTL EEFVSSGDLT 

       970 
DLYHTLWNDN TSYPFL 

References and documentation are available.

Number of amino acids: 976

Molecular weight: 112571.28

Theoretical pI: 6.14

Amino acid composition: 
Ala (A)  44	  4.5%
Arg (R)  39	  4.0%
Asn (N)  61	  6.2%
Asp (D)  52	  5.3%
Cys (C)  19	  1.9%
Gln (Q)  45	  4.6%
Glu (E)  60	  6.1%
Gly (G)  23	  2.4%
His (H)  21	  2.2%
Ile (I)  71	  7.3%
Leu (L) 108	 11.1%
Lys (K)  63	  6.5%
Met (M)  17	  1.7%
Phe (F)  55	  5.6%
Pro (P)  41	  4.2%
Ser (S)  98	 10.0%
Thr (T)  64	  6.6%
Trp (W)   9	  0.9%
Tyr (Y)  45	  4.6%
Val (V)  41	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 112
Total number of positively charged residues (Arg + Lys): 102

Atomic composition:

Carbon      C	      5068
Hydrogen    H	      7869
Nitrogen    N	      1313
Oxygen      O	      1514
Sulfur      S	        36

Formula: C5068H7869N1313O1514S36
Total number of atoms: 15800

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   117675
Abs 0.1% (=1 g/l)   1.045, assuming all pairs of Cys residues form cystines


Ext. coefficient   116550
Abs 0.1% (=1 g/l)   1.035, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.00
This classifies the protein as unstable.



Aliphatic index: 88.22

Grand average of hydropathicity (GRAVY): -0.308