ProtParam
User-provided sequence:
10 20 30 40 50 60
MKVLCVAEKN SIAKAVSQIL GGGRSTSRDS GYMYVKNYDF MFSGFPFARN GANCEVTMTS
70 80 90 100 110 120
VAGHLTGIDF SHDSHGWGKC AIQELFDAPL NEIMNNNQKK IASNIKREAR NADYLMIWTD
130 140 150 160 170 180
CDREGEYIGW EIWQEAKRGN RLIQNDQVYR AVFSHLERQH ILNAARNPSR LDMKSVHAVG
190 200 210 220 230 240
TRIEIDLRAG VTFTRLLTET LRNKLRNQAT MTKDGAKHRG GNKNDSQVVS YGTCQFPTLG
250 260 270 280 290 300
FVVDRFERIR NFVPEEFWYI QLVVENKDNG GTTTFQWDRG HLFDRLSVLT FYETCIETAG
310 320 330 340 350 360
NVAQVVDLKS KPTTKYRPLP LTTVELQKNC ARYLRLNAKQ SLDAAEKLYQ KGFISYPRTE
370 380 390 400 410 420
TDTFPHAMDL KSLVEKQAQL DQLAAGGRTA WASYAASLLQ PENTSNNNKF KFPRSGSHDD
430 440 450 460 470 480
KAHPPIHPIV SLGPEANVSP VERRVYEYVA RHFLACCSED AKGQSMTLVL DWAVERFSAS
490 500 510 520 530 540
GLVVLERNFL DVYPWARWET TKQLPRLEMN ALVDIAKAEM KAGTTAPPKP MTESELILLM
550 560 570 580 590 600
DTNGIGTDAT IAEHIDKIQV RNYVRSEKVG KETYLQPTTL GVSLVHGFEA IGLEDSFAKP
610 620 630 640 650
FQRREMEQDL KKICEGHASK TDVVKDIVEK YRKYWHKTNA CKNTLLQVYD RVKASM
References and
documentation are available.
Number of amino acids: 656
Molecular weight: 74370.57
Theoretical pI: 8.71
Total number of negatively charged residues (Asp + Glu): 79
Total number of positively charged residues (Arg + Lys): 87
Atomic composition:
Carbon C 3291
Hydrogen H 5174
Nitrogen N 934
Oxygen O 980
Sulfur S 27
Formula: C
3291H
5174N
934O
980S
27
Total number of atoms: 10406
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 93905
Abs 0.1% (=1 g/l) 1.263, assuming all pairs of Cys residues form cystines
Ext. coefficient 93280
Abs 0.1% (=1 g/l) 1.254, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.58
This classifies the protein as stable.
Aliphatic index: 77.32
Grand average of hydropathicity (GRAVY): -0.479