ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKVNMEYTKE KKVGEGTYAV VYLGCQHSTG RKIAIKEIKT SEFKDGLDMS AIREVKYLQE 

        70         80         90        100        110        120 
MQHPNVIELI DIFMAYDNLN LVLEFLPTDL EVVIKDKSIL FTPADIKAWM LMTLRGVYHC 

       130        140        150        160        170        180 
HRNFILHRDL KPNNLLFSPD GQIKVADFGL ARAIPAPHEI LTSNVVTRWY RAPELLFGAK 

       190        200        210        220        230        240 
HYTSAIDIWS VGVIFAELML RIPYLPGQND VDQMEVTFRA LGTPTDRDWP EVSSFMTYNK 

       250        260        270        280        290        300 
LQIYPPPSRD ELRKRFIAAS EYALDFMCGM LTMNPQKRWT AVQCLESDYF KELPPPSDPS 


SIKIRN 

References and documentation are available.

Number of amino acids: 306

Molecular weight: 35246.92

Theoretical pI: 6.40

Amino acid composition: 
Ala (A)  19	  6.2%
Arg (R)  16	  5.2%
Asn (N)  12	  3.9%
Asp (D)  19	  6.2%
Cys (C)   4	  1.3%
Gln (Q)   9	  2.9%
Glu (E)  19	  6.2%
Gly (G)  13	  4.2%
His (H)   7	  2.3%
Ile (I)  22	  7.2%
Leu (L)  30	  9.8%
Lys (K)  20	  6.5%
Met (M)  13	  4.2%
Phe (F)  14	  4.6%
Pro (P)  20	  6.5%
Ser (S)  16	  5.2%
Thr (T)  16	  5.2%
Trp (W)   5	  1.6%
Tyr (Y)  13	  4.2%
Val (V)  19	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1599
Hydrogen    H	      2494
Nitrogen    N	       414
Oxygen      O	       449
Sulfur      S	        17

Formula: C1599H2494N414O449S17
Total number of atoms: 4973

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    47120
Abs 0.1% (=1 g/l)   1.337, assuming all pairs of Cys residues form cystines


Ext. coefficient    46870
Abs 0.1% (=1 g/l)   1.330, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.85
This classifies the protein as stable.



Aliphatic index: 90.49

Grand average of hydropathicity (GRAVY): -0.198