ProtParam
User-provided sequence:
10 20 30 40 50 60
MKVQITNSRT EEILKVQANN ENDEVSKATP GEVEESLRLI GDLKFFLATA PVNWQENQII
70 80 90 100 110 120
RRYYLNSGQG FVSCVFWNNL YYITGTDIVK CCLYRMQKFG REVVQKKKFE EGIFSDLRNL
130 140 150 160 170 180
KCGIDATLEQ PKSEFLSFLF RNMCLKTQKK QKVFFWFSVA HDKLFADALE RDLKRESLNQ
190 200 210 220 230 240
PSTTKPVNEP ALSFSYDSSS DKPLYDQLLQ HLDSRRPSST TKSDNSPPKL ESENFKDNEL
250 260 270 280 290 300
VTVTNQPLLG VGLMDDDAPE SPSQINDFIP QKLIIEPNTL ELNGLTEETP HDLPKNTAKG
310 320 330 340 350 360
RDEEDFPLDY FPVSVEYPTE ENAFDPFPPQ AFTPAAPSMP ISYDNVNERD SMPVNSLLNR
370 380 390 400 410 420
YPYQLSVAPT FPVPPSSSRQ HFMTNRDFYS SNNNKEKLVS PSDPTSYMKY DEPVMDFDES
430 440 450 460 470 480
RPNENCTNAK SHNSGQQTKQ HQLYSNNFQQ SYPNGMVPGY YPKMPYNPMG GDPLLDQAFY
490 500 510 520 530 540
GADDFFFPPE GCDNNMLYPQ TATSWNVLPP QAMQPAPTYV GRPYTPNYRS TPGSAMFPYM
550 560 570 580 590 600
QSSNSMQWNT AVSPYSSRAP STTAKNYPPS TFYSQNINQY PRRRTVGMKS SQGNVPTGNK
610 620 630 640 650 660
QSVGKSAKIS KPLHIKTSAY QKQYKINLET KARPSAGDED SAHPDKNKEI SMPTPDSNTL
670 680
VVQSEEGGAH SLEVDTNRRS DKNLPDAT
References and
documentation are available.
Number of amino acids: 688
Molecular weight: 77866.75
Theoretical pI: 5.47
Total number of negatively charged residues (Asp + Glu): 83
Total number of positively charged residues (Arg + Lys): 71
Atomic composition:
Carbon C 3439
Hydrogen H 5264
Nitrogen N 928
Oxygen O 1089
Sulfur S 26
Formula: C
3439H
5264N
928O
1089S
26
Total number of atoms: 10746
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 75555
Abs 0.1% (=1 g/l) 0.970, assuming all pairs of Cys residues form cystines
Ext. coefficient 75180
Abs 0.1% (=1 g/l) 0.965, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 58.69
This classifies the protein as unstable.
Aliphatic index: 57.11
Grand average of hydropathicity (GRAVY): -0.819