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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKVQITNSRT EEILKVQANN ENDEVSKATP GEVEESLRLI GDLKFFLATA PVNWQENQII

70 80 90 100 110 120
RRYYLNSGQG FVSCVFWNNL YYITGTDIVK CCLYRMQKFG REVVQKKKFE EGIFSDLRNL

130 140 150 160 170 180
KCGIDATLEQ PKSEFLSFLF RNMCLKTQKK QKVFFWFSVA HDKLFADALE RDLKRESLNQ

190 200 210 220 230 240
PSTTKPVNEP ALSFSYDSSS DKPLYDQLLQ HLDSRRPSST TKSDNSPPKL ESENFKDNEL

250 260 270 280 290 300
VTVTNQPLLG VGLMDDDAPE SPSQINDFIP QKLIIEPNTL ELNGLTEETP HDLPKNTAKG

310 320 330 340 350 360
RDEEDFPLDY FPVSVEYPTE ENAFDPFPPQ AFTPAAPSMP ISYDNVNERD SMPVNSLLNR

370 380 390 400 410 420
YPYQLSVAPT FPVPPSSSRQ HFMTNRDFYS SNNNKEKLVS PSDPTSYMKY DEPVMDFDES

430 440 450 460 470 480
RPNENCTNAK SHNSGQQTKQ HQLYSNNFQQ SYPNGMVPGY YPKMPYNPMG GDPLLDQAFY

490 500 510 520 530 540
GADDFFFPPE GCDNNMLYPQ TATSWNVLPP QAMQPAPTYV GRPYTPNYRS TPGSAMFPYM

550 560 570 580 590 600
QSSNSMQWNT AVSPYSSRAP STTAKNYPPS TFYSQNINQY PRRRTVGMKS SQGNVPTGNK

610 620 630 640 650 660
QSVGKSAKIS KPLHIKTSAY QKQYKINLET KARPSAGDED SAHPDKNKEI SMPTPDSNTL

670 680
VVQSEEGGAH SLEVDTNRRS DKNLPDAT


References and documentation are available.

Number of amino acids: 688 Molecular weight: 77866.75 Theoretical pI: 5.47
Amino acid composition: 
Ala (A) 33 4.8% Arg (R) 27 3.9% Asn (N) 54 7.8% Asp (D) 42 6.1% Cys (C) 7 1.0% Gln (Q) 38 5.5% Glu (E) 41 6.0% Gly (G) 28 4.1% His (H) 9 1.3% Ile (I) 19 2.8% Leu (L) 48 7.0% Lys (K) 44 6.4% Met (M) 19 2.8% Phe (F) 33 4.8% Pro (P) 65 9.4% Ser (S) 68 9.9% Thr (T) 42 6.1% Trp (W) 5 0.7% Tyr (Y) 32 4.7% Val (V) 34 4.9% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 83 Total number of positively charged residues (Arg + Lys): 71 Atomic composition: Carbon C 3439 Hydrogen H 5264 Nitrogen N 928 Oxygen O 1089 Sulfur S 26 Formula: C3439H5264N928O1089S26 Total number of atoms: 10746 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 75555 Abs 0.1% (=1 g/l) 0.970, assuming all pairs of Cys residues form cystines Ext. coefficient 75180 Abs 0.1% (=1 g/l) 0.965, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 58.69 This classifies the protein as unstable. Aliphatic index: 57.11 Grand average of hydropathicity (GRAVY): -0.819