ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKVSDRRKFE KANFDEFESA LNNKNDLVHC PSITLFESIP TEVRSFYEDE KSGLIKVVKF 

        70         80         90        100        110        120 
RTGAMDRKRS FEKVVISVMV GKNVKKFLTF VEDEPDFQGG PIPSKYLIPK KINLMVYTLF 

       130        140        150        160        170        180 
QVHTLKFNRK DYDTLSLFYL NRGYYNELSF RVLERCHEIA SARPNDSSTM RTFTDFVSGA 

       190        200        210        220        230        240 
PIVRSLQKST IRKYGYNLAP YMFLLLHVDE LSIFSAYQAS LPGEKKVDTE RLKRDLCPRK 

       250        260        270        280        290        300 
PIEIKYFSQI CNDMMNKKDR LGDILHIILR ACALNFGAGP RGGAGDEEDR SITNEEPIIP 

       310        320        330        340        350        360 
SVDEHGLKVC KLRSPNTPRR LRKTLDAVKA LLVSSCACTA RDLDIFDDNN GVAMWKWIKI 

       370        380        390        400        410        420 
LYHEVAQETT LKDSYRITLV PSSDGISLLA FAGPQRNVYV DDTTRRIQLY TDYNKNGSSE 

       430        440        450        460        470        480 
PRLKTLDGLT SDYVFYFVTV LRQMQICALG NSYDAFNHDP WMDVVGFEDP NQVTNRDISR 

       490        500        510        520        530        540 
IVLYSYMFLN TAKGCLVEYA TFRQYMRELP KNAPQKLNFR EMRQGLIALG RHCVGSRFET 

       550        560        570        580        590        600 
DLYESATSEL MANHSVQTGR NIYGVDSFSL TSVSGTTATL LQERASERWI QWLGLESDYH 

       610        620        630        640        650        660 
CSFSSTRNAE DVVAGEAASS NHHQKISRVT RKRPREPKST NDILVAGQKL FGSSFEFRDL 

       670        680        690        700        710        720 
HQLRLCYEIY MADTPSVAVQ APPGYGKTEL FHLPLIALAS KGDVEYVSFL FVPYTVLLAN 

       730        740        750        760        770        780 
CMIRLGRCGC LNVAPVRNFI EEGYDGVTDL YVGIYDDLAS TNFTDRIAAW ENIVECTFRT 

       790        800        810        820        830        840 
NNVKLGYLIV DEFHNFETEV YRQSQFGGIT NLDFDAFEKA IFLSGTAPEA VADAALQRIG 

       850        860        870        880        890        900 
LTGLAKKSMD INELKRSEDL SRGLSSYPTR MFNLIKEKSE VPLGHVHKIR KKVESQPEEA 

       910        920        930        940        950        960 
LKLLLALFES EPESKAIVVA STTNEVEELA CSWRKYFRVV WIHGKLGAAE KVSRTKEFVT 

       970        980        990       1000       1010       1020 
DGSMQVLIGT KLVTEGIDIK QLMMVIMLDN RLNIIELIQG VGRLRDGGLC YLLSRKNSWA 

      1030       1040       1050       1060       1070       1080 
ARNRKGELPP IKEGCITEQV REFYGLESKK GKKGQHVGCC GSRTDLSADT VELIERMDRL 

      1090       1100       1110       1120       1130       1140 
AEKQATASMS IVALPSSFQE SNSSDRYRKY CSSDEDSNTC IHGSANASTN ASTNAITTAS 

      1150       1160       1170       1180       1190       1200 
TNVRTNATTN ASTNATTNAS TNASTNATTN ASTNATTNSS TNATTTASTN VRTSATTTAS 

      1210       1220       1230       1240       1250       1260 
INVRTSATTT ESTNSSTNAT TTESTNSSTN ATTTESTNSN TSATTTASIN VRTSATTTES 

      1270       1280       1290       1300       1310       1320 
TNSSTSATTT ASINVRTSAT TTKSINSSTN ATTTESTNSN TNATTTESTN SSTNATTTES 

      1330       1340       1350       1360       1370       1380 
TNSSTNATTT ESTNSNTSAA TTESTNSNTS ATTTESTNAS AKEDANKDGN AEDNRFHPVT 

      1390       1400       1410       1420       1430       1440 
DINKESYKRK GSQMVLLERK KLKAQFPNTS ENMNVLQFLG FRSDEIKHLF LYGIDIYFCP 

      1450       1460       1470       1480       1490       1500 
EGVFTQYGLC KGCQKMFELC VCWAGQKVSY RRIAWEALAV ERMLRNDEEY KEYLEDIEPY 

      1510       1520       1530       1540       1550       1560 
HGDPVGYLKY FSVKRREIYS QIQRNYAWYL AITRRRETIS VLDSTRGKQG SQVFRMSGRQ 

      1570       1580       1590       1600       1610       1620 
IKELYFKVWS NLRESKTEVL QYFLNWDEKK CQEEWEAKDD TVVVEALEKG GVFQRLRSMT 

      1630       1640       1650       1660       1670       1680 
SAGLQGPQYV KLQFSRHHRQ LRSRYELSLG MHLRDQIALG VTPSKVPHWT AFLSMLIGLF 

      1690       1700       1710       1720       1730       1740 
YNKTFRQKLE YLLEQISEVW LLPHWLDLAN VEVLAADDTR VPLYMLMVAV HKELDSDDVP 

      1750 
DGRFDILLCR DSSREVGE 

References and documentation are available.

Number of amino acids: 1758

Molecular weight: 197511.63

Theoretical pI: 7.30

Amino acid composition: 
Ala (A) 121	  6.9%
Arg (R) 111	  6.3%
Asn (N) 103	  5.9%
Asp (D)  89	  5.1%
Cys (C)  31	  1.8%
Gln (Q)  53	  3.0%
Glu (E) 122	  6.9%
Gly (G)  89	  5.1%
His (H)  30	  1.7%
Ile (I)  82	  4.7%
Leu (L) 154	  8.8%
Lys (K) 100	  5.7%
Met (M)  35	  2.0%
Phe (F)  71	  4.0%
Pro (P)  51	  2.9%
Ser (S) 163	  9.3%
Thr (T) 163	  9.3%
Trp (W)  18	  1.0%
Tyr (Y)  63	  3.6%
Val (V) 109	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 211
Total number of positively charged residues (Arg + Lys): 211

Atomic composition:

Carbon      C	      8659
Hydrogen    H	     13703
Nitrogen    N	      2425
Oxygen      O	      2726
Sulfur      S	        66

Formula: C8659H13703N2425O2726S66
Total number of atoms: 27579

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   194745
Abs 0.1% (=1 g/l)   0.986, assuming all pairs of Cys residues form cystines


Ext. coefficient   192870
Abs 0.1% (=1 g/l)   0.976, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.93
This classifies the protein as stable.



Aliphatic index: 77.22

Grand average of hydropathicity (GRAVY): -0.431