ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKWENYCDFV AGCFGGACGV LVAHPLDTIK VWQQASNSSV VTAIQQIYSR NNGVNGFYRG 

        70         80         90        100        110        120 
MFFPFISTGA INSLLFGIYG NHLRQLRKVC HSDYQREQLE YHNMFLAGSV AGFVQSFIAC 

       130        140        150        160        170        180 
PMELIKVRLQ TATYYSDYLY GQRRTAFGTF KRILKTDGIS GLYRGLLPMM CRDVLPYGIY 

       190        200        210        220        230        240 
MLAYRQGVDY MDRRDFVRRR RSQSDGSSVN LLVTTLAGAW AGVISWVCVI PFDVVKTLMQ 

       250        260        270        280        290        300 
ADENHKYRGI FHCVRVQYRA YGWRSIFRGS WMLVARAVPF NAATFLGYEY ALEWCQRWNG 


TYV 

References and documentation are available.

Number of amino acids: 303

Molecular weight: 34809.17

Theoretical pI: 9.44

Amino acid composition: 
Ala (A)  22	  7.3%
Arg (R)  25	  8.3%
Asn (N)  12	  4.0%
Asp (D)  12	  4.0%
Cys (C)   9	  3.0%
Gln (Q)  15	  5.0%
Glu (E)   7	  2.3%
Gly (G)  27	  8.9%
His (H)   6	  2.0%
Ile (I)  15	  5.0%
Leu (L)  24	  7.9%
Lys (K)   8	  2.6%
Met (M)  10	  3.3%
Phe (F)  18	  5.9%
Pro (P)   7	  2.3%
Ser (S)  18	  5.9%
Thr (T)  13	  4.3%
Trp (W)   8	  2.6%
Tyr (Y)  21	  6.9%
Val (V)  26	  8.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 33

Atomic composition:

Carbon      C	      1581
Hydrogen    H	      2391
Nitrogen    N	       433
Oxygen      O	       421
Sulfur      S	        19

Formula: C1581H2391N433O421S19
Total number of atoms: 4845

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    75790
Abs 0.1% (=1 g/l)   2.177, assuming all pairs of Cys residues form cystines


Ext. coefficient    75290
Abs 0.1% (=1 g/l)   2.163, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.79
This classifies the protein as stable.



Aliphatic index: 82.34

Grand average of hydropathicity (GRAVY): -0.015