ProtParam
User-provided sequence:
10 20 30 40 50 60
MKYILVTGGV ISGIGKGVIA SSVGTILKSC GLHVTSIKID PYINIDAGTF SPYEHGEVFV
70 80 90 100 110 120
LDDGGEVDLD LGNYERFLDI RLTKDNNLTT GKIYQYVINK ERKGDYLGKT VQVVPHITDA
130 140 150 160 170 180
IQEWVMRQAL IPVDEDGLEP QVCVIELGGT VGDIESMPFI EAFRQFQFKV KRENFCNIHV
190 200 210 220 230 240
SLVPQPSSTG EQKTKPTQNS VRELRGLGLS PDLVVCRCSN PLDTSVKEKI SMFCHVEPEQ
250 260 270 280 290 300
VICVHDVSSI YRVPLLLEEQ GVVDYFLRRL DLPIERQSRK MLMKWKEMAD RYDRLLETCS
310 320 330 340 350 360
IALVGKYTKF SDSYASVIKA LEHSALAINH KLEIKYIDST DLEPSTLQEE PVRYHEAWQK
370 380 390 400 410 420
LCSAHGVLVP GGFGVRGTEG KIQAIAWARK QKKPFLGVCL GMQLAVVEFS RNVLGWQDAN
430 440 450 460 470 480
STEFDPKTSH PVVIDMPEHN PGQMGGTMRL GKRRTLFQTK NSVMRKLYGD TDYLEERHRH
490 500 510 520 530 540
RFEVNPVLKK CLEEQGLKFV GQDVEGERME IVELEDHPFF VGVQYHPEFL SRPIKPSPPY
550 560 570 580 590
FGLLLASVGR LPHYLQKGCR LSPRDTYSDR SGSSSPDSEI TELKFPSISQ D
References and
documentation are available.
Number of amino acids: 591
Molecular weight: 66682.46
Theoretical pI: 6.14
Total number of negatively charged residues (Asp + Glu): 79
Total number of positively charged residues (Arg + Lys): 71
Atomic composition:
Carbon C 2976
Hydrogen H 4702
Nitrogen N 808
Oxygen O 880
Sulfur S 25
Formula: C
2976H
4702N
808O
880S
25
Total number of atoms: 9391
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 58050
Abs 0.1% (=1 g/l) 0.871, assuming all pairs of Cys residues form cystines
Ext. coefficient 57300
Abs 0.1% (=1 g/l) 0.859, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.83
This classifies the protein as unstable.
Aliphatic index: 88.78
Grand average of hydropathicity (GRAVY): -0.310