ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG 

        70         80         90        100        110        120 
GRKHVAALRT VKSLVDNMIT GVTKGYKYKM RYVYAHFPIN VNIVEKDGAK FIEVRNFLGD 

       130        140        150        160        170        180 
KKIRNVPVRD GVTIEFSTNV KDEIVLSGNS VEDVSQNAAD LQQICRVRNK DIRKFLDGIY 

       190 
VSHKGFITED L 

References and documentation are available.

Number of amino acids: 191

Molecular weight: 21569.02

Theoretical pI: 9.73

Amino acid composition: 
Ala (A)   7	  3.7%
Arg (R)  11	  5.8%
Asn (N)  13	  6.8%
Asp (D)  11	  5.8%
Cys (C)   1	  0.5%
Gln (Q)   7	  3.7%
Glu (E)   9	  4.7%
Gly (G)  13	  6.8%
His (H)   5	  2.6%
Ile (I)  17	  8.9%
Leu (L)  10	  5.2%
Lys (K)  20	 10.5%
Met (M)   3	  1.6%
Phe (F)   7	  3.7%
Pro (P)   4	  2.1%
Ser (S)   8	  4.2%
Thr (T)  12	  6.3%
Trp (W)   0	  0.0%
Tyr (Y)   6	  3.1%
Val (V)  27	 14.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 20
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	       963
Hydrogen    H	      1576
Nitrogen    N	       274
Oxygen      O	       278
Sulfur      S	         4

Formula: C963H1576N274O278S4
Total number of atoms: 3095

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     8940
Abs 0.1% (=1 g/l)   0.414, assuming all pairs of Cys residues form cystines


Ext. coefficient     8940
Abs 0.1% (=1 g/l)   0.414, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.97
This classifies the protein as stable.



Aliphatic index: 99.79

Grand average of hydropathicity (GRAVY): -0.259