ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKYKIFDTHA HYDSDSFDED RENVIKELQE NGVIGVLNCG SDLYGLRKSV ELAKEFDMFY 

        70         80         90        100        110        120 
AAVGIHPENA DEFNEDVVKE IKEFVKNEKV KAIGEIGLDY YWEENPPREV QKEVFRAQMK 

       130        140        150        160        170        180 
LADELNLPVV IHDRDAHKDT LEIMKEFPNV IGVVHCFSGS VEFAKECIKL GYYIGFTGVL 

       190        200        210        220        230        240 
TFKNAKKLVD VCREIPAERM LVETDCPFMA PVPFRGKRNK SDYIEYIIDK MSEIRGISGE 

       250 
EMNEILLNNK KRLFKI 

References and documentation are available.

Number of amino acids: 256

Molecular weight: 29591.94

Theoretical pI: 5.12

Amino acid composition: 
Ala (A)  13	  5.1%
Arg (R)  11	  4.3%
Asn (N)  14	  5.5%
Asp (D)  18	  7.0%
Cys (C)   5	  2.0%
Gln (Q)   3	  1.2%
Glu (E)  30	 11.7%
Gly (G)  15	  5.9%
His (H)   6	  2.3%
Ile (I)  20	  7.8%
Leu (L)  17	  6.6%
Lys (K)  25	  9.8%
Met (M)   8	  3.1%
Phe (F)  15	  5.9%
Pro (P)   9	  3.5%
Ser (S)   9	  3.5%
Thr (T)   5	  2.0%
Trp (W)   1	  0.4%
Tyr (Y)  10	  3.9%
Val (V)  22	  8.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1329
Hydrogen    H	      2074
Nitrogen    N	       344
Oxygen      O	       394
Sulfur      S	        13

Formula: C1329H2074N344O394S13
Total number of atoms: 4154

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    20650
Abs 0.1% (=1 g/l)   0.698, assuming all pairs of Cys residues form cystines


Ext. coefficient    20400
Abs 0.1% (=1 g/l)   0.689, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.86
This classifies the protein as unstable.



Aliphatic index: 86.37

Grand average of hydropathicity (GRAVY): -0.385