ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKYNATKAIS IAPPTIPTVN PTINEVFSFE LLELELEEEE DKLLSCESDC FAQYVDGHLS 

        70         80         90        100        110        120 
HVNEVITQYS PEAQTGHDAV ESQTTHPSLP LVAEVEEVAV LDKDELAEAL EELDIFCLIF 


RKRT 

References and documentation are available.

Number of amino acids: 124

Molecular weight: 13892.55

Theoretical pI: 4.21

Amino acid composition: 
Ala (A)  10	  8.1%
Arg (R)   2	  1.6%
Asn (N)   4	  3.2%
Asp (D)   7	  5.6%
Cys (C)   3	  2.4%
Gln (Q)   4	  3.2%
Glu (E)  19	 15.3%
Gly (G)   2	  1.6%
His (H)   4	  3.2%
Ile (I)   7	  5.6%
Leu (L)  14	 11.3%
Lys (K)   5	  4.0%
Met (M)   1	  0.8%
Phe (F)   5	  4.0%
Pro (P)   7	  5.6%
Ser (S)   8	  6.5%
Thr (T)   9	  7.3%
Trp (W)   0	  0.0%
Tyr (Y)   3	  2.4%
Val (V)  10	  8.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 7

Atomic composition:

Carbon      C	       616
Hydrogen    H	       963
Nitrogen    N	       151
Oxygen      O	       205
Sulfur      S	         4

Formula: C616H963N151O205S4
Total number of atoms: 1939

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     4595
Abs 0.1% (=1 g/l)   0.331, assuming all pairs of Cys residues form cystines


Ext. coefficient     4470
Abs 0.1% (=1 g/l)   0.322, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.77
This classifies the protein as unstable.



Aliphatic index: 97.50

Grand average of hydropathicity (GRAVY): -0.168