ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKYNIVHGIC LLFAITQAVG AVHFYAKSGE TKCFYEHLSR GNLLIGDLDL YVEKDGLFEE 

        70         80         90        100        110        120 
DPESSLTITV DETFDNDHRV LNQKNSHTGD VTFTALDTGE HRFCFTPFYS KKSATLRVFI 

       130        140        150        160        170        180 
ELEIGNVEAL DSKKKEDMNS LKGRVGQLTQ RLSSIRKEQD AIREKEAEFR NQSESANSKI 

       190        200        210 
MTWSVFQLLI LLGTCAFQLR YLKNFFVKQK VV 

References and documentation are available.

Number of amino acids: 212

Molecular weight: 24248.63

Theoretical pI: 6.32

Amino acid composition: 
Ala (A)  11	  5.2%
Arg (R)  10	  4.7%
Asn (N)  10	  4.7%
Asp (D)  12	  5.7%
Cys (C)   4	  1.9%
Gln (Q)   9	  4.2%
Glu (E)  17	  8.0%
Gly (G)  12	  5.7%
His (H)   6	  2.8%
Ile (I)  11	  5.2%
Leu (L)  23	 10.8%
Lys (K)  17	  8.0%
Met (M)   3	  1.4%
Phe (F)  15	  7.1%
Pro (P)   2	  0.9%
Ser (S)  15	  7.1%
Thr (T)  14	  6.6%
Trp (W)   1	  0.5%
Tyr (Y)   6	  2.8%
Val (V)  14	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 27

Atomic composition:

Carbon      C	      1085
Hydrogen    H	      1703
Nitrogen    N	       291
Oxygen      O	       325
Sulfur      S	         7

Formula: C1085H1703N291O325S7
Total number of atoms: 3411

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14690
Abs 0.1% (=1 g/l)   0.606, assuming all pairs of Cys residues form cystines


Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.595, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.34
This classifies the protein as stable.



Aliphatic index: 86.89

Grand average of hydropathicity (GRAVY): -0.301