ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKYPAFSINS DTVHLTDNPL DDYQRLYLVS VLDRDSPPAS FSAGLNIRKV NYKSSIAAQF 

        70         80         90        100        110        120 
THPNFIISAR DAGNGEEAAA QNVLNCFEYQ FPNLQTIQSL VHEQTLLSQL ASSATPHSAL 

       130        140        150        160        170        180 
HLHDKNILMG KIILPSRSNK TPVSASPTKQ EKKALSTASR ENATSSLTKN QQFKLTKMDH 

       190        200        210        220        230        240 
NLINDKLINP NNCVIWSHDS GYVFMTGIWR LYQDVMKGLI NLPRGDSVST SQQQFFCKAE 

       250        260        270        280        290        300 
FEKILSFCFY NHSSFTSEES SSVLLSSSTS SPPKRRTSTG STFLDANASS SSTSSTQANN 

       310        320        330        340        350        360 
YIDFHWNNIK PELRDLICQS YKDFLINELG PDQIDLPNLN PANFTKRIRG GYIKIQGTWL 

       370        380        390        400        410        420 
PMEISRLLCL RFCFPIRYFL VPIFGPDFPK DCESWYLAHQ NVTFASSTTG AGAATAATAA 

       430        440        450        460        470        480 
ANTSTNFTST AVARPRQKPR PRPRQRSTSM SHSKAQKLVI EDALPSFDSF VENLGLSSND 

       490        500        510        520        530        540 
KNFIKKNSKR QKSSTYTSQT SSPIGPRDPT VQILSNLASF YNTHGHRYSY PGNIYIPQQR 

       550        560        570        580        590        600 
YSLPPPNQLS SPQRQLNYTY DHIHPVPSQY QSPRHYNVPS SPIAPAPPTF PQPYGDDHYH 

       610        620        630        640 
FLKYASEVYK QQNQRPAHNT NTNMDTSFSP RANNSLNNFK FKTNSKQ 

References and documentation are available.

Number of amino acids: 647

Molecular weight: 72687.39

Theoretical pI: 9.39

Amino acid composition: 
Ala (A)  42	  6.5%
Arg (R)  30	  4.6%
Asn (N)  51	  7.9%
Asp (D)  29	  4.5%
Cys (C)   8	  1.2%
Gln (Q)  38	  5.9%
Glu (E)  17	  2.6%
Gly (G)  21	  3.2%
His (H)  20	  3.1%
Ile (I)  34	  5.3%
Leu (L)  51	  7.9%
Lys (K)  35	  5.4%
Met (M)   8	  1.2%
Phe (F)  34	  5.3%
Pro (P)  49	  7.6%
Ser (S)  83	 12.8%
Thr (T)  44	  6.8%
Trp (W)   5	  0.8%
Tyr (Y)  27	  4.2%
Val (V)  21	  3.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 65

Atomic composition:

Carbon      C	      3226
Hydrogen    H	      4970
Nitrogen    N	       906
Oxygen      O	       983
Sulfur      S	        16

Formula: C3226H4970N906O983S16
Total number of atoms: 10101

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    68230
Abs 0.1% (=1 g/l)   0.939, assuming all pairs of Cys residues form cystines


Ext. coefficient    67730
Abs 0.1% (=1 g/l)   0.932, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.47
This classifies the protein as unstable.



Aliphatic index: 67.14

Grand average of hydropathicity (GRAVY): -0.604