ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKYVLVTGGV VSGLGKGVTA SSIGVLLKAC GLRVTSIKID PYLNTDAGTM SPFEHGEVFV 

        70         80         90        100        110        120 
LDDGGEVDLD LGNYERFLDI KLTRDNNITT GKIYQHVIAK ERKGDYLGKT VQVVPHVTDA 

       130        140        150        160        170        180 
IQDWIERVAV IPVDGEEDPA DVCVIELGGT IGDIESAPFI EALGQFSYRV GPGNFCLVHV 

       190        200        210        220        230        240 
SLVPVLNVVG EQKTKPTQHS VKGLRGLGLT PDILACRSTK PLEDNVKEKL AQFCHVPLEY 

       250        260        270        280        290        300 
IFTLYDVPNI WRIPLLLKDQ KAHLAISKVL NLASILNEPS LGEWTSRAEL CDNLHVPVRI 

       310        320        330        340        350        360 
AVVGKYTGLS DAYLSVLKAL LHASVACRKK LVVDWVPACD LEKETEKENP DAYKAAWKLL 

       370        380        390        400        410        420 
KGVDGILVPG GFGDRGVEGK ILAAKYAREN KIPFLGICLG MQIAVIEFAR SVLSLQDANS 

       430        440        450        460        470        480 
TEFNPETKHP CIIFMPEGSK THMGGTMRLG SRKSIFNVKD SKSAKLYENK SFVDERHRHR 

       490        500        510        520        530        540 
YEVNPDMVER LEKAGLSFAA KDETGKRMEI IELPNHPFFI GAQFHPEFKS RPGKASPLFL 

       550        560        570        580        590        600 
GLIAASCGEL DTVLNPASAH QHSISNGPTN IFINGTSKKS PNGLADVRYN NGYCNGLYTR 

References and documentation are available.

Number of amino acids: 600

Molecular weight: 65728.67

Theoretical pI: 6.79

Amino acid composition: 
Ala (A)  41	  6.8%
Arg (R)  25	  4.2%
Asn (N)  27	  4.5%
Asp (D)  33	  5.5%
Cys (C)  12	  2.0%
Gln (Q)  12	  2.0%
Glu (E)  38	  6.3%
Gly (G)  53	  8.8%
His (H)  17	  2.8%
Ile (I)  37	  6.2%
Leu (L)  62	 10.3%
Lys (K)  44	  7.3%
Met (M)   8	  1.3%
Phe (F)  22	  3.7%
Pro (P)  32	  5.3%
Ser (S)  35	  5.8%
Thr (T)  28	  4.7%
Trp (W)   5	  0.8%
Tyr (Y)  17	  2.8%
Val (V)  52	  8.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 71
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      2948
Hydrogen    H	      4687
Nitrogen    N	       797
Oxygen      O	       862
Sulfur      S	        20

Formula: C2948H4687N797O862S20
Total number of atoms: 9314

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    53580
Abs 0.1% (=1 g/l)   0.815, assuming all pairs of Cys residues form cystines


Ext. coefficient    52830
Abs 0.1% (=1 g/l)   0.804, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.54
This classifies the protein as stable.



Aliphatic index: 96.32

Grand average of hydropathicity (GRAVY): -0.115