ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKYVVVSGGV ISGIGKGVLA SSTGMLMKTL GLKVTSIKID PYMNIDAGTM SPLEHGECFV 

        70         80         90        100        110        120 
LDDGGETDLD LGNYERYLGV TLTKDHNITT GKIYSHVIAK ERKGDYLGKT VQIVPHLTNA 

       130        140        150        160        170        180 
IQDWIERVAK IPVDDTGMEP DVCIIELGGT VGDIESAPFV EALRQFQFKV GKENFALIHV 

       190        200        210        220        230        240 
SLVPVIHGEQ KTKPTQAAIK GLRSLGLVPD MIACRCSETL DKPTIDKIAM FCHVGPEQVV 

       250        260        270        280        290        300 
NVHDVNSTYH VPLLLLEQKM IDYLHARLKL DEISLTEEEK QRGLELLSKW KATTGNFDES 

       310        320        330        340        350        360 
METVKIALVG KYTNLKDSYL SVIKALEHSS MKCRRKLDIK WVEATDLEPE AQESNKTKFH 

       370        380        390        400        410        420 
EAWNMVSTAD GILIPGGFGV RGTEGMVLAA RWARENHIPF LGVCLGLQIA TIEFTRSVLG 

       430        440        450        460        470        480 
RKDSHSAEFY PDIDEKNHVV VFMPEIDKET MGGSMRLGLR PTFFQNETEW SQIKKLYGDV 

       490        500        510        520        530        540 
SEVHERHRHR YEINPKMVDE LENNGLIFVG KDDTGKRCEI LELKNHPYYI ATQYHPEYTS 

       550        560        570 
KVLDPSKPFL GLVAASAGIL QDVIEGKYDL EAGENKFNF 

References and documentation are available.

Number of amino acids: 579

Molecular weight: 64710.28

Theoretical pI: 5.67

Amino acid composition: 
Ala (A)  30	  5.2%
Arg (R)  21	  3.6%
Asn (N)  20	  3.5%
Asp (D)  35	  6.0%
Cys (C)   8	  1.4%
Gln (Q)  15	  2.6%
Glu (E)  48	  8.3%
Gly (G)  48	  8.3%
His (H)  20	  3.5%
Ile (I)  40	  6.9%
Leu (L)  55	  9.5%
Lys (K)  45	  7.8%
Met (M)  17	  2.9%
Phe (F)  19	  3.3%
Pro (P)  23	  4.0%
Ser (S)  30	  5.2%
Thr (T)  35	  6.0%
Trp (W)   6	  1.0%
Tyr (Y)  18	  3.1%
Val (V)  46	  7.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 83
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2890
Hydrogen    H	      4580
Nitrogen    N	       768
Oxygen      O	       864
Sulfur      S	        25

Formula: C2890H4580N768O864S25
Total number of atoms: 9127

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60320
Abs 0.1% (=1 g/l)   0.932, assuming all pairs of Cys residues form cystines


Ext. coefficient    59820
Abs 0.1% (=1 g/l)   0.924, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.87
This classifies the protein as stable.



Aliphatic index: 92.21

Grand average of hydropathicity (GRAVY): -0.239