ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLAASFKRQP SQLVRGLGAV LRTPTRIGHV RTMATLKTTD KKAPEDIEGS DTVQIELPES 

        70         80         90        100        110        120 
SFESYMLEPP DLSYETSKAT LLQMYKDMVI IRRMEMACDA LYKAKKIRGF CHLSVGQEAI 

       130        140        150        160        170        180 
AVGIENAITK LDSIITSYRC HGFTFMRGAS VKAVLAELMG RRAGVSYGKG GSMHLYAPGF 

       190        200        210        220        230        240 
YGGNGIVGAQ VPLGAGLAFA HQYKNEDACS FTLYGDGASN QGQVFESFNM AKLWNLPVVF 

       250        260        270        280        290        300 
CCENNKYGMG TAASRSSAMT EYFKRGQYIP GLKVNGMDIL AVYQASKFAK DWCLSGKGPL 

       310        320        330        340        350        360 
VLEYETYRYG GHSMSDPGTT YRTRDEIQHM RSKNDPIAGL KMHLIDLGIA TEAEVKAYDK 

       370        380        390        400        410        420 
SARKYVDEQV ELADAAPPPE AKLSILFEDV YVKGTETPTL RGRIPEDTWD FKKQGFASRD 

References and documentation are available.

Number of amino acids: 420

Molecular weight: 46343.03

Theoretical pI: 8.29

Amino acid composition: 
Ala (A)  40	  9.5%
Arg (R)  22	  5.2%
Asn (N)  10	  2.4%
Asp (D)  22	  5.2%
Cys (C)   7	  1.7%
Gln (Q)  14	  3.3%
Glu (E)  26	  6.2%
Gly (G)  39	  9.3%
His (H)   8	  1.9%
Ile (I)  20	  4.8%
Leu (L)  33	  7.9%
Lys (K)  29	  6.9%
Met (M)  17	  4.0%
Phe (F)  16	  3.8%
Pro (P)  18	  4.3%
Ser (S)  28	  6.7%
Thr (T)  24	  5.7%
Trp (W)   3	  0.7%
Tyr (Y)  21	  5.0%
Val (V)  23	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 51

Atomic composition:

Carbon      C	      2055
Hydrogen    H	      3226
Nitrogen    N	       558
Oxygen      O	       614
Sulfur      S	        24

Formula: C2055H3226N558O614S24
Total number of atoms: 6477

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48165
Abs 0.1% (=1 g/l)   1.039, assuming all pairs of Cys residues form cystines


Ext. coefficient    47790
Abs 0.1% (=1 g/l)   1.031, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.08
This classifies the protein as stable.



Aliphatic index: 74.62

Grand average of hydropathicity (GRAVY): -0.297