ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLAMARKKLS LRRKESRKWR EPPEKALRFE NVIIQDTNLP FEQVEESKKM LGDALQLCGI 

        70         80         90        100        110 
ENEIASFMKR KFDAKYGGHW QCVVGRNFGS HLDPIQFIHF TVSKISVILF R 

References and documentation are available.

Number of amino acids: 111

Molecular weight: 13009.24

Theoretical pI: 9.74

Amino acid composition: 
Ala (A)   6	  5.4%
Arg (R)   9	  8.1%
Asn (N)   4	  3.6%
Asp (D)   4	  3.6%
Cys (C)   2	  1.8%
Gln (Q)   5	  4.5%
Glu (E)   9	  8.1%
Gly (G)   6	  5.4%
His (H)   3	  2.7%
Ile (I)   8	  7.2%
Leu (L)  10	  9.0%
Lys (K)  11	  9.9%
Met (M)   4	  3.6%
Phe (F)   8	  7.2%
Pro (P)   4	  3.6%
Ser (S)   7	  6.3%
Thr (T)   2	  1.8%
Trp (W)   2	  1.8%
Tyr (Y)   1	  0.9%
Val (V)   6	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 13
Total number of positively charged residues (Arg + Lys): 20

Atomic composition:

Carbon      C	       586
Hydrogen    H	       934
Nitrogen    N	       166
Oxygen      O	       157
Sulfur      S	         6

Formula: C586H934N166O157S6
Total number of atoms: 1849

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12615
Abs 0.1% (=1 g/l)   0.970, assuming all pairs of Cys residues form cystines


Ext. coefficient    12490
Abs 0.1% (=1 g/l)   0.960, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.66
This classifies the protein as unstable.



Aliphatic index: 84.32

Grand average of hydropathicity (GRAVY): -0.395