ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLANDSATTD SEVSTPPSSR KDLADGVAEG DNDNDIDTGD TDANADEDVD AAAAIALEAE 

        70         80         90        100        110        120 
EREELQQFIS ELREKIESKR QLRLQNSTIE LPGEEYFAKL DSNLKKNTAF VKKLKLFTAT 

       130        140        150        160        170        180 
QLDGLLREIS ALNLSKYISE ICAALVEAKL KMTDVPAVVT LASRLHCTYA DFDVHFLEAW 

       190        200        210        220        230        240 
QKALNIKATE KIGNPSKLRV DLRLFAELVS SGVIQMKPGL AQLGVVLVHL IALDKDDHSN 

       250        260        270        280        290        300 
FSIILSFCRH CGEEYAGLVP QKMQQLATKY GVEVPKSDFL TADKQLNLRT MLKGYFKALC 

       310        320        330        340        350        360 
KHVLAEQAEL MNMTKNIRRT MECKGEISTE KREKCELMQA GFDKLLASAQ SLSELLGEPL 

       370        380        390        400        410        420 
PELAKESEGC NPGTVIDNML DSASFGVLDP WGDEETRAFY TDLPDLRQFL PNFSAPKVDL 

       430        440        450        460        470        480 
ETLEEPSELT EEAIDANLDA EMDLDDPPST TSDTTPENPI EEQPTTPVAA AEDLKPQKMG 

       490        500        510        520        530        540 
NALMELGRQQ QQQSQLNQNP IQIQNQMRQQ FDGFLVNLFN CVNKELIDSA AIEFLLNFNT 

       550        560        570        580        590        600 
KHQRKKLTRT IFSVQRTRLD ILPYLSRFVA IVHMCNTDVA TDLAELLRKE FKWHIHKKNQ 

       610        620        630        640        650        660 
LNIETKLKIV RFIGELVKFG LFKKFDALGC LKMLLRDFQH HQIEMACAFV EVTGVYLYNC 

       670        680        690        700        710        720 
RDARLLMNVF LDQMLRLKTA TAMDSRHAAQ IESVYYLVKP PESSKREPMV RPAMHEYIRY 

       730        740        750        760        770        780 
LIFEELCKQN VDRCIKMLRR IDWQDPETNC YAIKCLSKAY LLRFQLIRCL ADLVSGLSSY 

       790        800        810        820        830        840 
QPRAVTIVID NVFEDIRAGL EIHSPRMAQR RIAMAKYLGE MYNYKLVEST NILNTLYSII 

       850        860        870        880        890        900 
SLGVSNDQNV VSPLDPPDSL FRLKLACMLL DTCAPYFTSQ ATRKKLDYFL VFFQHYYWFK 

       910        920        930        940        950        960 
KSHPVFSKTE NTSDLFPILM DHTYRDCLLN VRPKLKIYKS LEQAKAAIDH LQEKLYPQLK 

       970        980        990       1000       1010       1020 
TTNNAQDPSL GTISEISEID EGGTDEDSGS SNDQRERQVS GQEQDQSNDW TENEAEPPLP 

      1030       1040       1050       1060       1070       1080 
PLPPPEKSKE DLEFEQLYEK MTTDSYQERL KEPIKATAKD IPVPMMARLQ KKSYDQITGA 

      1090       1100       1110       1120       1130       1140 
QTIGPGINNM SQISKSVPNQ DGTPGSPGAD SPDGNGKSGG GVGGSDVGAA GAAGASGATS 

      1150       1160       1170       1180       1190       1200 
VPFVLMVRGH KGGKQQFKSF VAPSDSHLAT NLKRQEQMIR EEKEKVKRLT LNITERIEEE 

      1210       1220       1230       1240 
DYQESLMPPQ HRNFTQSYYQ KPNKHKFKHQ KGAPDADLIF H 

References and documentation are available.

Number of amino acids: 1241

Molecular weight: 140056.60

Theoretical pI: 5.46

Amino acid composition: 
Ala (A)  93	  7.5%
Arg (R)  57	  4.6%
Asn (N)  56	  4.5%
Asp (D)  83	  6.7%
Cys (C)  21	  1.7%
Gln (Q)  70	  5.6%
Glu (E)  96	  7.7%
Gly (G)  57	  4.6%
His (H)  25	  2.0%
Ile (I)  62	  5.0%
Leu (L) 138	 11.1%
Lys (K)  91	  7.3%
Met (M)  35	  2.8%
Phe (F)  49	  3.9%
Pro (P)  62	  5.0%
Ser (S)  80	  6.4%
Thr (T)  66	  5.3%
Trp (W)   6	  0.5%
Tyr (Y)  34	  2.7%
Val (V)  60	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 179
Total number of positively charged residues (Arg + Lys): 148

Atomic composition:

Carbon      C	      6182
Hydrogen    H	      9835
Nitrogen    N	      1685
Oxygen      O	      1906
Sulfur      S	        56

Formula: C6182H9835N1685O1906S56
Total number of atoms: 19664

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    84910
Abs 0.1% (=1 g/l)   0.606, assuming all pairs of Cys residues form cystines


Ext. coefficient    83660
Abs 0.1% (=1 g/l)   0.597, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.30
This classifies the protein as unstable.



Aliphatic index: 84.37

Grand average of hydropathicity (GRAVY): -0.453