ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLAWLRHRIR SYNTSTYSSI LPSASFGKVY KIGTKLNFTL LALCLLLACS VFFNYFYLAD 

        70         80         90        100        110        120 
NNGLDIDTKG EEEENVFKDR KMVIFPNNFE ITDKNLLEYY LKTLEEPLHP QDTIYRNRFI 

       130        140        150        160        170        180 
YKVPDVSYTS QTINLFSGLS QNSQSSKCED LSSSYSFDVS GPQNKNCDLY KVLGKFLNDN 

       190        200        210        220        230        240 
SEYFQEISPL FPKLKEMLVK KEIEKHWFQL IGSSVWLEQY GVHLMTSRIF YSSTGDKVKP 

       250        260        270        280        290        300 
VVSLTYVQVF DHEWREIENV ELIVPDGEGK YKPMTYPTFL PMSVYHNEKQ QQGRFYGVED 

       310        320        330        340        350        360 
PRITLVRNKL GYDEPIIVYN SHHRKITDAK SDNDGESNIH FKAYRSIFMA WLWQNQKGKN 

       370        380        390        400        410        420 
NVEEIETGKM KNRVYVKSKE LIKPNNKRED KEKNWAPFIN YQQRLQQGFD SHVYFMYQFQ 

       430        440        450        460        470        480 
DLKILKCSLL DEEDCVWEYQ FNDKNGAGRL RGGTELVNIN QLLTTFDHPE IKRVKDLMPQ 

       490        500        510        520        530        540 
NREIWIGVAR AALEKCGCGD KMYRPNIVIL IKDGDDQYRL SHVSPFVGLG ILILPWWPDK 

       550        560        570        580        590        600 
GLCDGKNLII PNGISSWHLN KDEDNSVQDY LTLSISRADS TVDLLHIKGL LKSILFDDPN 

       610        620        630        640        650 
SKLLELNDYG FNNKNIECAV KSSDAFCKKY GSEYKLNNNK EEDKANGNGK GSSS 

References and documentation are available.

Number of amino acids: 654

Molecular weight: 75828.12

Theoretical pI: 6.57

Amino acid composition: 
Ala (A)  17	  2.6%
Arg (R)  24	  3.7%
Asn (N)  50	  7.6%
Asp (D)  43	  6.6%
Cys (C)  11	  1.7%
Gln (Q)  25	  3.8%
Glu (E)  43	  6.6%
Gly (G)  38	  5.8%
His (H)  14	  2.1%
Ile (I)  41	  6.3%
Leu (L)  66	 10.1%
Lys (K)  59	  9.0%
Met (M)  11	  1.7%
Phe (F)  31	  4.7%
Pro (P)  25	  3.8%
Ser (S)  51	  7.8%
Thr (T)  23	  3.5%
Trp (W)  12	  1.8%
Tyr (Y)  34	  5.2%
Val (V)  36	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 86
Total number of positively charged residues (Arg + Lys): 83

Atomic composition:

Carbon      C	      3418
Hydrogen    H	      5262
Nitrogen    N	       900
Oxygen      O	      1010
Sulfur      S	        22

Formula: C3418H5262N900O1010S22
Total number of atoms: 10612

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   117285
Abs 0.1% (=1 g/l)   1.547, assuming all pairs of Cys residues form cystines


Ext. coefficient   116660
Abs 0.1% (=1 g/l)   1.538, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.73
This classifies the protein as unstable.



Aliphatic index: 82.37

Grand average of hydropathicity (GRAVY): -0.552