ProtParam
User-provided sequence:
10 20 30 40 50 60
MLCAISGKVP RRPVLSPKSR TIFEKSLLEQ YVKDTGNDPI TNEPLSIEEI VEIVPSAQQA
70 80 90 100 110 120
SLTESTNSAT LKANYSIPNL LTSLQNEWDA IMLENFKLRS TLDSLTKKLS TVMYERDAAK
130 140 150 160 170 180
LVAAQLLMEK NEDSKDLPKS SQQAVAITRE EFLQGLLQSS RDFVARGKLK APKWPILKNL
190 200 210 220 230 240
ELLQAQNYSR NIKTFPYKEL NKSMYYDKWV CMCRCEDGAL HFTQLKDSKT ITTITTPNPR
250 260 270 280 290 300
TGGEHPAIIS RGPCNRLLLL YPGNQITILD SKTNKVLREI EVDSANEIIY MYGHNEVNTE
310 320 330 340 350 360
YFIWADNRGT IGFQSYEDDS QYIVHSAKSD VEYSSGVLHK DSLLLALYSP DGILDVYNLS
370 380 390 400 410 420
SPDQASSRFP VDEEAKIKEV KFADNGYWMV VECDQTVVCF DLRKDVGTLA YPTYTIPEFK
430 440 450 460 470 480
TGTVTYDIDD SGKNMIAYSN ESNSLTIYKF DKKTKNWTKD EESALCLQSD TADFTDMDVV
490 500
CGDGGIAAIL KTNDSFNIVA LTP
References and
documentation are available.
Number of amino acids: 503
Molecular weight: 56570.06
Theoretical pI: 5.03
Total number of negatively charged residues (Asp + Glu): 70
Total number of positively charged residues (Arg + Lys): 55
Atomic composition:
Carbon C 2508
Hydrogen H 3958
Nitrogen N 654
Oxygen O 793
Sulfur S 19
Formula: C
2508H
3958N
654O
793S
19
Total number of atoms: 7932
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 66280
Abs 0.1% (=1 g/l) 1.172, assuming all pairs of Cys residues form cystines
Ext. coefficient 65780
Abs 0.1% (=1 g/l) 1.163, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.27
This classifies the protein as unstable.
Aliphatic index: 85.88
Grand average of hydropathicity (GRAVY): -0.387