ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLCQQMIRTT AKRSSNIMTR PIIMKRSVHF KDGVYENIPF KVKGRKTPYA LSHFGFFAIG 

        70 
FAVPFVACYV QLKKSGAF 

References and documentation are available.

Number of amino acids: 78

Molecular weight: 8906.66

Theoretical pI: 10.42

Amino acid composition: 
Ala (A)   6	  7.7%
Arg (R)   5	  6.4%
Asn (N)   2	  2.6%
Asp (D)   1	  1.3%
Cys (C)   2	  2.6%
Gln (Q)   3	  3.8%
Glu (E)   1	  1.3%
Gly (G)   5	  6.4%
His (H)   2	  2.6%
Ile (I)   6	  7.7%
Leu (L)   3	  3.8%
Lys (K)   8	 10.3%
Met (M)   4	  5.1%
Phe (F)   8	 10.3%
Pro (P)   4	  5.1%
Ser (S)   5	  6.4%
Thr (T)   4	  5.1%
Trp (W)   0	  0.0%
Tyr (Y)   3	  3.8%
Val (V)   6	  7.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 2
Total number of positively charged residues (Arg + Lys): 13

Atomic composition:

Carbon      C	       410
Hydrogen    H	       644
Nitrogen    N	       110
Oxygen      O	       100
Sulfur      S	         6

Formula: C410H644N110O100S6
Total number of atoms: 1270

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     4595
Abs 0.1% (=1 g/l)   0.516, assuming all pairs of Cys residues form cystines


Ext. coefficient     4470
Abs 0.1% (=1 g/l)   0.502, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.37
This classifies the protein as unstable.



Aliphatic index: 75.00

Grand average of hydropathicity (GRAVY): 0.073