ProtParam
User-provided sequence:
10 20 30 40 50 60
MLCQQMIRTT AKRSSNIMTR PIIMKRSVHF KDGVYENIPF KVKGRKTPYA LSHFGFFAIG
70
FAVPFVACYV QLKKSGAF
References and
documentation are available.
Number of amino acids: 78
Molecular weight: 8906.66
Theoretical pI: 10.42
Total number of negatively charged residues (Asp + Glu): 2
Total number of positively charged residues (Arg + Lys): 13
Atomic composition:
Carbon C 410
Hydrogen H 644
Nitrogen N 110
Oxygen O 100
Sulfur S 6
Formula: C
410H
644N
110O
100S
6
Total number of atoms: 1270
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 4595
Abs 0.1% (=1 g/l) 0.516, assuming all pairs of Cys residues form cystines
Ext. coefficient 4470
Abs 0.1% (=1 g/l) 0.502, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.37
This classifies the protein as unstable.
Aliphatic index: 75.00
Grand average of hydropathicity (GRAVY): 0.073