ProtParam
User-provided sequence:
10 20 30 40 50 60
MLCRLVGNRS LGARYTASIK AIACYSTSGK QRNGKIYESA IDAVADVQDG AQILFGGFGI
70 80 90 100 110 120
CGIPEKMINA LKQKGVKNIT GVSNNGGVDD TGLGVLIKQK QVSKVIGSYV GENTELVRQY
130 140 150 160 170 180
LEGELAVELT PQGTLAEKIR AGGAGIPAFY TPTGYATLVQ EGGAPIKYSK DGKVEISSEK
190 200 210 220 230 240
KPVKEFNGKN YVMEESIFAD FAFVKAQKAD PLGNLVFNKA ARNFNAPMCR AAKITVAEVE
250 260 270 280 290 300
EIVPIGALSP DEIHVPGIYI NRIFKGTNYN KRVERLRITE PKDPSKPAPP PNPAQVLRER
310 320 330 340 350 360
IARRVALEFH DGMYANLGIG IPVLSSNYIP KGMNVMLQSE NGILGLGPFP TKDKVDPDLI
370 380 390 400 410 420
NAGKESVTVV PGASYFGSDD SFAMIRGGHV DITILGAMEV SATGDLANWM IPGKLVKGMG
430 440 450 460 470 480
GAMDLVAAPG TKVIITMEHN ARDGSPKILD TCSLPLTGKG VIDMIISEKA VFTVEKGVGL
490 500 510
TLIEVAEGYT VDDIIASTGA KFTVSPNLKK MGQIPV
References and
documentation are available.
Number of amino acids: 516
Molecular weight: 54904.47
Theoretical pI: 8.53
Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 58
Atomic composition:
Carbon C 2446
Hydrogen H 3955
Nitrogen N 657
Oxygen O 731
Sulfur S 20
Formula: C
2446H
3955N
657O
731S
20
Total number of atoms: 7809
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28100
Abs 0.1% (=1 g/l) 0.512, assuming all pairs of Cys residues form cystines
Ext. coefficient 27850
Abs 0.1% (=1 g/l) 0.507, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 28.12
This classifies the protein as stable.
Aliphatic index: 93.35
Grand average of hydropathicity (GRAVY): -0.036