ProtParam
User-provided sequence:
10 20 30 40 50 60
MLCTGSNAST IIQEQKHQQD SASADHRSTL LGLETELKKD SRSLLFSCCC LALAWAAALP
70 80 90 100 110 120
RASAAARSAL TLASAFTLSL ASTAAFSVFS LASAYAPALL CFCETFGSLK GYEVENHMNA
130 140 150 160 170 180
SVTMLEEAIM YSSFSSKKRI RHSSSLTNDL IEEILSRLHS KSVARFRCVS KQCASMFASP
190 200 210 220 230 240
YFKKLFQTRS SAKPSLLFAI ADYGIEEDYS MKFFSSPQLE NTYEKTSSTL VAAAEFHVKF
250 260 270 280 290 300
SPDKSIPIYL SEDPRYVSIG YASGLIYNYC GRYVGRPLIC NPNTGRYAIL PYRYTYRKAY
310 320 330 340 350 360
SFFGFDPIDK QYKALSLPYV DGPGRSKVLT FGAGDLTWRN IKSHTEDISY RYYEYVIVCF
370 380 390 400 410 420
DVTSEKFTFV GVQKFCLLIN YKGKLDVIYW EDDVDIHEIC YFAKDLDEYL DENLDADATN
430 440 450 460 470 480
ELHVWVLEDV EKQEWSKYAY TWTDDTFFHR HLSIAGATAS GEIVFSMRKY TSKQPFYVFY
490 500 510 520 530
FNPERNTLQR VEIQGFGEAF RTTCSVRTFV NHIEDLDVND LEQLNYIGTR R
References and
documentation are available.
Number of amino acids: 531
Molecular weight: 60428.42
Theoretical pI: 6.10
Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 56
Atomic composition:
Carbon C 2731
Hydrogen H 4144
Nitrogen N 702
Oxygen O 809
Sulfur S 21
Formula: C
2731H
4144N
702O
809S
21
Total number of atoms: 8407
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 84535
Abs 0.1% (=1 g/l) 1.399, assuming all pairs of Cys residues form cystines
Ext. coefficient 83660
Abs 0.1% (=1 g/l) 1.384, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.35
This classifies the protein as stable.
Aliphatic index: 79.23
Grand average of hydropathicity (GRAVY): -0.193