ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLDANLKNQL QTYLQNLKRP VELVVSADES KKSQELKSLV NDIVSLSTFV SLKEAQGQRT 

        70         80         90        100        110        120 
PAMTVVNPAL NTQISFAGLP MGHEFTSLVL ALLHSGGHPI KLSDDIIEQI RNLPGKYEFE 

       130        140        150        160        170        180 
TYVSLTCQNC PDVVQALNMM AAINPNITNV MIDGALFQDE VASRNIMAVP SVYLNGEVFA 

       190        200        210        220        230        240 
AGRISIGEIL NKLDTGAASR KAEELSQKAP YEVLVVGGGP AGAAAAIYAA RKGLRTGIVA 

       250        260        270        280        290        300 
DKFGGQVAET VGIENFISVK ATEGPKLVAN LEAHVRDYEV DIMDNQKAVK LASDGLFELE 

       310        320        330        340        350        360 
LASGAKLRSR TVLLATGARW REMNVPGEKE YRGKGVAYCP HCDGPLFKGK RVAVIGGGNS 

       370        380        390        400        410        420 
GIEAAIDLAN IVEHVTVLEF DSKLRADDVL QRKAASMGNI KIITQAMTTE VTGDGTRVNG 

       430        440        450        460        470        480 
LNYTDRATGE SHHIALAGIF VQIGLVPNAE WLKGTVDLTP RGEIIVDERG QTSVPGVFAA 

       490        500        510        520 
GDVTNSPYKQ IIIAMGSGAN ASLGAFDYLI RHSDDSTETK TTDTKAA 

References and documentation are available.

Number of amino acids: 527

Molecular weight: 56262.15

Theoretical pI: 5.53

Amino acid composition: 
Ala (A)  58	 11.0%
Arg (R)  22	  4.2%
Asn (N)  27	  5.1%
Asp (D)  28	  5.3%
Cys (C)   4	  0.8%
Gln (Q)  19	  3.6%
Glu (E)  33	  6.3%
Gly (G)  48	  9.1%
His (H)   9	  1.7%
Ile (I)  34	  6.5%
Leu (L)  48	  9.1%
Lys (K)  29	  5.5%
Met (M)  12	  2.3%
Phe (F)  14	  2.7%
Pro (P)  19	  3.6%
Ser (S)  31	  5.9%
Thr (T)  32	  6.1%
Trp (W)   2	  0.4%
Tyr (Y)  12	  2.3%
Val (V)  46	  8.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 51

Atomic composition:

Carbon      C	      2476
Hydrogen    H	      4006
Nitrogen    N	       688
Oxygen      O	       771
Sulfur      S	        16

Formula: C2476H4006N688O771S16
Total number of atoms: 7957

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    29130
Abs 0.1% (=1 g/l)   0.518, assuming all pairs of Cys residues form cystines


Ext. coefficient    28880
Abs 0.1% (=1 g/l)   0.513, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 24.87
This classifies the protein as stable.



Aliphatic index: 97.00

Grand average of hydropathicity (GRAVY): -0.047