ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLDFVVIFTK GGVVLWHSNA SGNSFASCIN SLIRGVILEE RNTEAKYYEE DHLAVQFKLD 

        70         80         90        100        110        120 
NELDLVYAAI FQKVIKLNYL DGFLADMQAA FKEKYGDIRL GDDYDFDREY RRVLSAAEEA 

       130        140        150        160        170        180 
SAKQVKAPKT MRSYNESQKS KKTVASMIQD DKKPVEKRVN IQEAPPPSKS QPSSPPTGSP 

       190        200        210        220        230        240 
MDKIIMEKRR KLREKLTPTK KTSPSDSKSS KPEKAGKKPR VWDLGGNSKD AALLDRSRDS 

       250        260        270        280        290        300 
PDDVQYQNIN SELVGTMQGV IRDLDVESED EADNEDASSE GEAEEQVQSK KGKRGGLLSY 

       310        320        330        340        350        360 
FKGIVGAKTM SLADLQPALE KMRDHLISKN VASEIAAKLC DSVAASLDGK QMGTFDSIAS 

       370        380        390        400        410        420 
QVKEALTESL VRILSPKRRI DIIRDALESK RNGRPYTIIF CGVNGVGKST NLAKICFWLI 

       430        440        450        460        470        480 
ENDFNVLIAA CDTFRAGAVE QLRTHTRHLN ALHPAAKHDG RNMVQLYEKG YGKDAAGIAM 

       490        500        510        520        530        540 
EAIKFAHDTR VDVVLVDTAG RMQDNEPLMR SLSKLIKVNN PDLVLFVGEA LVGNEAVDQL 

       550        560        570        580        590        600 
VKFNQSLADY SSNENPHIID GIVLTKFDTI DDKVGAAISM TYITGQPIVF VGTGQTYADL 

       610 
KAINVNAVVN SLMK 

References and documentation are available.

Number of amino acids: 614

Molecular weight: 67795.13

Theoretical pI: 6.38

Amino acid composition: 
Ala (A)  55	  9.0%
Arg (R)  30	  4.9%
Asn (N)  30	  4.9%
Asp (D)  49	  8.0%
Cys (C)   5	  0.8%
Gln (Q)  23	  3.7%
Glu (E)  38	  6.2%
Gly (G)  38	  6.2%
His (H)   9	  1.5%
Ile (I)  37	  6.0%
Leu (L)  51	  8.3%
Lys (K)  54	  8.8%
Met (M)  16	  2.6%
Phe (F)  19	  3.1%
Pro (P)  22	  3.6%
Ser (S)  48	  7.8%
Thr (T)  25	  4.1%
Trp (W)   3	  0.5%
Tyr (Y)  16	  2.6%
Val (V)  46	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 87
Total number of positively charged residues (Arg + Lys): 84

Atomic composition:

Carbon      C	      2975
Hydrogen    H	      4802
Nitrogen    N	       832
Oxygen      O	       931
Sulfur      S	        21

Formula: C2975H4802N832O931S21
Total number of atoms: 9561

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40590
Abs 0.1% (=1 g/l)   0.599, assuming all pairs of Cys residues form cystines


Ext. coefficient    40340
Abs 0.1% (=1 g/l)   0.595, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.68
This classifies the protein as stable.



Aliphatic index: 86.58

Grand average of hydropathicity (GRAVY): -0.400