ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLDLLRLQLT TFIMNDVPTP YACYFQDSAT PNQEGILELH DNIMFYLLVI LGLVSWMLYT 

        70         80         90        100        110        120 
IVMTYSKNPI AYKYIKHGQT IEVIWTIFPA VILLIIAFPS FILLYLCDEV ISPAMTIKAI 

       130        140        150        160        170        180 
GYQWYWKYEY SDFINDSGET VEFESYVIPD ELLEEGQLRL LDTDTSMVVP VDTHIRFVVT 

       190        200        210        220        230        240 
AADVIHDFAI PSLGIKVDAT PGRLNQVSAL IQREGVFYGA CSELCGTGHA NMPIKIEAVS 

       250 
LPKFLEWLNE Q 

References and documentation are available.

Number of amino acids: 251

Molecular weight: 28567.20

Theoretical pI: 4.45

Amino acid composition: 
Ala (A)  15	  6.0%
Arg (R)   5	  2.0%
Asn (N)   8	  3.2%
Asp (D)  14	  5.6%
Cys (C)   4	  1.6%
Gln (Q)   9	  3.6%
Glu (E)  16	  6.4%
Gly (G)  12	  4.8%
His (H)   5	  2.0%
Ile (I)  26	 10.4%
Leu (L)  29	 11.6%
Lys (K)   8	  3.2%
Met (M)   8	  3.2%
Phe (F)  12	  4.8%
Pro (P)  13	  5.2%
Ser (S)  13	  5.2%
Thr (T)  16	  6.4%
Trp (W)   5	  2.0%
Tyr (Y)  14	  5.6%
Val (V)  19	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 13

Atomic composition:

Carbon      C	      1324
Hydrogen    H	      2026
Nitrogen    N	       306
Oxygen      O	       372
Sulfur      S	        12

Formula: C1324H2026N306O372S12
Total number of atoms: 4040

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48610
Abs 0.1% (=1 g/l)   1.702, assuming all pairs of Cys residues form cystines


Ext. coefficient    48360
Abs 0.1% (=1 g/l)   1.693, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.19
This classifies the protein as stable.



Aliphatic index: 113.39

Grand average of hydropathicity (GRAVY): 0.353