ProtParam
User-provided sequence:
10 20 30 40 50 60
MLDSLVSKLP SLSTSDHASV VALNLFVALL CACIVLGHLL EENRWMNESI TALLIGLGTG
70 80 90 100 110 120
VTILLISKGK SSHLLVFSED LFFIYLLPPI IFNAGFQVKK KQFFRNFVTI MLFGAVGTII
130 140 150 160 170 180
SCTIISLGVT QFFKKLDIGT FDLGDYLAIG AIFAATDSVC TLQVLNQDET PLLYSLVFGE
190 200 210 220 230 240
GVVNDATSVV VFNAIQSFDL THLNHEAAFH LLGNFLYLFL LSTLLGAATG LISAYVIKKL
250 260 270 280 290 300
YFGRHSTDRE VALMMLMAYL SYMLAELFDL SGILTVFFCG IVMSHYTWHN VTESSRITTK
310 320 330 340 350 360
HTFATLSFLA ETFIFLYVGM DALDIDKWRS VSDTPGTSIA VSSILMGLVM VGRAAFVFPL
370 380 390 400 410 420
SFLSNLAKKN QSEKINFNMQ VVIWWSGLMR GAVSMALAYN KFTRAGHTDV RGNAIMITST
430 440 450 460 470 480
ITVCLFSTVV FGMLTKPLIS YLLPHQNATT SMLSDDNTPK SIHIPLLDQD SFIEPSGNHN
490 500 510 520 530
VPRPDSIRGF LTRPTRTVHY YWRQFDDSFM RPVFGGRGFV PFVPGSPTER NPPDLSKA
References and
documentation are available.
Number of amino acids: 538
Molecular weight: 59513.42
Theoretical pI: 6.73
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 37
Atomic composition:
Carbon C 2738
Hydrogen H 4252
Nitrogen N 680
Oxygen O 753
Sulfur S 24
Formula: C
2738H
4252N
680O
753S
24
Total number of atoms: 8447
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 54235
Abs 0.1% (=1 g/l) 0.911, assuming all pairs of Cys residues form cystines
Ext. coefficient 53860
Abs 0.1% (=1 g/l) 0.905, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.71
This classifies the protein as stable.
Aliphatic index: 106.71
Grand average of hydropathicity (GRAVY): 0.458